Abstract: Objective: To study the effect of lemon essential oil (LEO) on the adhesion of main pathogenic bacteria of periodontal disease, to explore its feasibility for the prevention and treatment of periodontal disease. Methods: Effect on the smooth polystyrene surface: The bacteria liquid and experimental groups of each component in the liquid mix were cultured in the 96 well plate for 48h. Bacteria adhesion was tested protein coated polystyrene adhesion surface: The bacteria solution and experimental group of each component in the liquid mix were cultured in the protein coated 96 well plates for 48h, Bacteria adhesion was tested. Results: Effect of smooth polystyrene surface adhesion: coneerning to Aa, Pi, there was no statistical significance between the LEO group and the control group; coneerning to Pg, Fn, there was significant difference between the LEO group and the control group (P<0.05); Protein coated polystyrene adhesion surface: coneerning to Aa, Pg, Pi, Fn, there was significant difference between LEO group and the control group (P<0.05). LEO could inhibit the adhesion of Aa, Pg, Pi, Fn on the protein coated surface, can inhibit the adhesion of Pg, Fn on the smooth surface. Conclusion: LEO can block the adhesion of pathogenic bacteria on the teeth surface, block the formation of the dendal plaque , and can prevent periodontal disease.

Key words: LEO, Periodontal disease, Pathogen, Adhesion