Effect of Different Frequency Tensile Stress on the Proliferation and Differentiation of Mandibular Condylar Chondrocytes

Abstract

Abstract: Objective: To investigate the effect of different frequency of tensile stress on the proliferation and differentiation of mandibular condylar chondrocytes. Methods: Mandibular condylar chondrocytes (MCC) from two-week-old New Zealand rabbits were cultured in vitro under different frequency(0.1Hz,0.5Hz,1Hz) tensile stress for a period of 2 h per day for 3 days .The proliferation of articular chondrocyte was determined by CCK-8 assay. The gene expression (Sox9, Runx2, VEGF )was analyzed using real-time RT-PCR. Results: The static(0HZ) tensile stress had a significant stimulate on the chondrocyte proliferation compared with periodic tensile stress(0.1-1HZ)and the control group. The expression of Sox9, Runx2 and VEGF under 0.5HZ and 1HZ was significantly higher than the static(0HZ) tensile stress and the control group. Conclusion: There are different effects on the proliferation and differentiation of MCC under different frequency tensile stress.

Key words: Endochondral ossification, Periodic tensile stress, Static tensile stress, Proliferation, Differentiation

CLC Number:

R783.5

WANG Ya-fei, WANG Si-han, GUO Xiao-kai, et al. Effect of Different Frequency Tensile Stress on the Proliferation and Differentiation of Mandibular Condylar Chondrocytes[J]. Journal of Oral Science Research, 2015, 31(7): 662-665.

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