Effect of Modified Platelet-rich Plasma on the Mineralization of Human Bone Marrow Stromal Cells

Abstract

Abstract: Objevtive: To investigate the effect of platelet-rich plasma (PRP) at different concentrations on the mineralization of human bone marrow stromal cells. Methods: BMSCs from healthy donor were cultured and passaged for 3-4 passages. PRP were manufactured from venous blood of the same donor. The activity of alkaline phosphatase in BMSCs was detemined using alkaline phosphatase reagent kit on the 7th day after mineralization induction. The formation of mineralized nodules in BMSCs were observed by alizarin red staining on the 21th days after minralization induction. After 7 days of minralization induction the expression of gene Runx2 in BMSCs was measured by real-time PCR. Results: PRP at the concentration of 5% evidently promoted the alkain phosphatase activity and the formation of mineralized nodules in BMSCs. The expression of gene Runx-2 was also evidently enhanced. Conclusion: PRP at the concentration of 5% could promote the mineralization of BMSCs.

Key words: PRP, BMSCs, Mineralization

CLC Number:

R781.4

LI Shi-han, DUAN Jian-min, WEN Jun, et al. Effect of Modified Platelet-rich Plasma on the Mineralization of Human Bone Marrow Stromal Cells[J]. Journal of Oral Science Research, 2015, 31(7): 735-738.

References

[1] Albanese A, et al. Platelet-rich plasma (PRP) in dental and oral surgery: from the wound healing to bone regeneration [J]. Immun Ageing, 2013, 10(1)∶23
[2] Zhang ZY, et al. The potential use of allogeneic platelet-rich plasma for large bone defect treatment: immunogenicity and defect healing efficacy [J]. Cell Transplant, 2013, 22(1)∶175-87
[3] 李洪涛,段建民,张宏斌,等.冻融洗涤血小板促进人牙髓细胞增殖的作用[J].牙体牙髓牙周病学杂志,2010,20(6)∶314-316
[4] 李洪涛,段建民,片山直. 富血小板血浆与洗涤血小板对人牙髓细胞矿化的作用[J].中国组织工程研究,2012,16(7)∶1206-1210
[5] Ko EK, et al. In vitro osteogenic differentiation of human mesenchymal stem cells and in vivo bone formation in composite nanofiber meshes [J]. Tissue Eng Part A, 2008, 14(12)∶2105-19
[6] Huang S, et al. Osteogenic differentiation of muscle satellite cells induced by platelet-rich plasma encapsulated in three-dimensional alginate scaffold [J]. Oral Surg Oral Med Oral Pathol Oral Radiol, 2012, 114(5)∶S32-40
[7] Liu T, et al. CD44+/CD105+ human amniotic fluid mesenchymal stem cells survive and proliferate in the ovary long-term in a mouse model of chemotherapy-induced premature ovarian failure [J]. Int J Med Sci, 2012, 9(7)∶592-602
[8] Sanchez AR, Sheridan PJ, Kupp LI. Is platelet-rich plasma the perfect enhancement factor? A current review [J]. Int J Oral Maxillofac Implants, 2003, 18(1)∶93-103
[9] Nagata MJ, et al. Effect of platelet-rich plasma on bone healing of autogenous bone grafts in critical-size defects [J]. J Clin Periodontol, 2009, 36(9)∶775-83
[10] Torigoe I, et al. Bone regeneration with autologous plasma, bone marrow stromal cells, and porous beta-tricalcium phosphate in nonhuman primates [J]. Tissue Eng Part A, 2009, 15(7)∶1489-99
[11] 李云鹏,赵昕,封兴华.人脐带间充质干细胞的原代培养、鉴定及成骨分化的实验研究[J].中国组织工程研究,2014,8(7)∶106-113

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