Differentially Expressed MicroRNA during Cementoblasts Mineralization Based Microarray.

doi:10.13701/j.cnki.kqyxyj.2017.02.005

Abstract

Abstract: Objective: To establish the mineralization cell model of cementoblasts and to study the microRNA expression profile during the differentiation and mineralization of cementoblasts. Methods: Mice cementoblast cell line OCCM30 was cultured in vitro and the cementoblasts were differentiated and mineralized in the presence of ascorbic acid, β-glycerophosphate, and dexamethasone. Then, the mineralization related markers ALP and OC were assessed by qPCR. Finally, the differentiated miRNA expression profile during the differentiation and mineralization of cementoblasts was screened by miRNA microarray and the accuracy of the results was verified by qPCR. Results: Cementoblasts were successfully induced to differentiation and mineralization. MiRNA microarray showed that there were 4 miRNAs were up-regulated and 39 miRNAs were down-regulated during this process. QPCR confirmed the accuracy of miRNA microarray. Conclusion: The cementoblast mineralization model was established successfully. MiRNA microarray discovered that the miRNAs were differently expressed during the mineralization of cementoblasts. This indicated that these miRNAs may have certain roles during the differentiation and mineralization of cementoblasts.

Key words: Cementoblast , Mineralization , MicroRNA, Microarray

CLC Number:

R781.4

WANG Chuan, LIAO Hai-qing, CAO Zheng-guo.. Differentially Expressed MicroRNA during Cementoblasts Mineralization Based Microarray.[J]. Journal of Oral Science Research, 2017, 33(2): 135-139.

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