Effects of Abrogation of Neuraminidase in Porphyromonas Gingivalis on Inflammation-related MicroRNA Expressions after Infected with Gingival Epithelial Cells.

doi:10.13701/j.cnki.kqyxyj.2017.02.004

Abstract

Abstract: Objective: To investigate effects of abrogation of neuraminidase in porphyromonas gingivalis on inflammation-related microRNA expressions after infected with gingival epithelial cells. Methods: P. gingivalis W83 and PG0352 mutant strain (△PG0352) were cultured anaerobically, and co-cultured with gingival epithelial cells at the MOI of 100:1. Inflammation-related microRNAs were detected by real-time PCR. Results: The expressions of hsa-miR-9, hsa-miR-23a, hsa-miR-23b, and hsa-miR-410 were significantly higher in △PG0352 group than those in P. gingivalis W83 group. The fold changes were 4.3387, 3.3978, 4.7930 and 4.3137, respectively. Conclusion: Neuraminidase deficient strain showed different effects on inflammation-related microRNA expression in gingival epithelial cells compared with wild-type strain.

Key words: Porphyromonas gingivalis, W83 , PG0352 , Gingival epithelial cell , MicroRNA

CLC Number:

R781.4

LI Chen, YANG Xue, PAN Ya-ping, XU Xiao-yu, TONG Tong, GUO Yan. Effects of Abrogation of Neuraminidase in Porphyromonas Gingivalis on Inflammation-related MicroRNA Expressions after Infected with Gingival Epithelial Cells.[J]. Journal of Oral Science Research, 2017, 33(2): 132-134.

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