Protein Kinase C Mediates Transforming Growth Factor β-induced integrin β6 Expression in Human Oral Squamous Cancer Cells

Abstract

Abstract: Objective: To investigate whether protein kinase C (PKC) is involved in the expression of integrin β6 by human oral squamous cancer cells (SAS) after transforming growth factor β (TGF-β) stimulation. Methods: Human oral squamous cancer cells SAS were cultured with DMEM with 10% fetal bovine serum, after TGF-β stimulation, integrin β6 protein and mRNA levels in the cell lysate were measured by western-blot and quantitative real-time PCR, respectively. Effect of PKC inhibition on integrin β6 expression was investigated by using PKC inhibitors. Results: TGF-β induced SAS integrin β6 mRNA and protein expression levels in a time-dependent manner. These effects of TGF-β could be attenuated by the broad spectrum PKC inhibitor RO-31-8220 in a concentration-dependent manner, whereas Ca²⁺-dependent PKC inhibitor G6976 had no effect. Conclusion: Ca²⁺-independent PKC mediates TGF-β-induced integrin β6 expression in cultured SAS cells.

Key words: Transforming growth factor β, Protein kinase C inhibitor Integrin β6, Human oral squamous cancer cells

CLC Number:

R739.8

GU Yin-zheng, DENG Xiao-ling, LI Qi-fu, et al. Protein Kinase C Mediates Transforming Growth Factor β-induced integrin β6 Expression in Human Oral Squamous Cancer Cells[J]. Journal of Oral Science Research, 2015, 31(7): 684-687.

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