Analysis of mRNA Expression of Exosomes Derived from Oral Squamous Cell Carcinoma.

doi:10.13701/j.cnki.kqyxyj.2018.09.006

Abstract

Abstract: Objective: To compare the mRNA expression between the exosomes derived from oral squamous cell cancer cells (OSCC) and human immortalized oral epithelial cell (HOEC), and to explore the evolutionary mechanism of OSCC by the approach of bioinformatic analysis. Methods: High throughput sequencing technology was used to explore mRNA expression profile in the exosomes of OSCC and HOEC. The differently expressed genes were selected and analyzed by Gene Ontoloty (GO) analysis and KEGG pathway analysis. Results: Among 44699 genes detected in the exosomes of OSCC and HOEC,471 differently expressed mRNAs were found (234 mRNAs were up-regulated and 237 mRNAs were down-regulated). Bioinformatics analysis showed that differently expressed mRNAs played an important role in the biological regulation and pathway of OSCC. Conclusion: Abundant exosomes can be secreted by OSCC and HOEC. The mRNA expression profiles of exosome from OSCC are quite different from those of HOEC, which play an important role in the occurrence, development, and metastasis of tumor. Exploring mRNA expression profiles of the exosomes derived from two different cells can provide new ideas and methods for understanding the evolutional mechanism of oral squamous cell cancer.

Key words: Oral cancer , Exosome , mRNA , High throughput sequencing technology

HU Hui-zhen, WANG Shi-qin, SUN Gui-jun, JIN Jia-jia, LI Zhi-yong, HE Xiang-yi. Analysis of mRNA Expression of Exosomes Derived from Oral Squamous Cell Carcinoma.[J]. Journal of Oral Science Research, 2018, 34(9): 943-947.

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