Comparison of Oral Microbial Diversity in Healthy People and Patients with Dental Caries and Periodontal Disease.

doi:10.13701/j.cnki.kqyxyj.2016.12.010

Abstract

Abstract: Objective: To compare the microbial diversity of healthy people and oral patients with high-throughput sequencing method and to provide basic data for curing dental caries and periodontal diseases. Methods: The patients with typical dental caries and periodontal diseases were selected. Their stimulated whole saliva and oropharyngeal samples were collected for high-throughput sequencing analysis and the microbial diversity among samples were analyzed using bioinformatics. Results: The Venn results indicated that a mean of 315 operational taxonomic units (OTUs) was obtained, and 73, 64,53, 19 and 18 common OTUs belonging to Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria and Fusobacteria were identified in healthy people. In addition, the relative richness results indicated that the Firmicute was reduced in children group and increased in youth group and adult group, while the Proteobacteria was increased in children group but reduced in youth group and adult group. Conclusion: The age bracket and oral disease had largely influenced the tooth development and microbial development in oral cavity, and the traditional "pathogenic bacteria" of Firmicutes, Proteobacteriaand Bacteroidetes might exert beneficial effects on oral health.

Key words: High-throughput sequencing analyses, Dental caries, Periodontal disease, Microbial diversity

SHI Yan, LI Chen-jie, ZHANG Ming-zheng, WANG Xin, YANG Shao-Guo, MENG Fan-Jin, CHEN Ting-Tao, YANG Jian.. Comparison of Oral Microbial Diversity in Healthy People and Patients with Dental Caries and Periodontal Disease.[J]. Journal of Oral Science Research, 2016, 32(12): 1265-1268.

References

[1] Palmer RJ. Composition and development of oral bacterial communities [J]. Periodontology, 2014,64(1)∶20-39
[2] Sturgeon A, Stull JW, Costa MC, et al. Metagenomic analysis of the canine oral cavity as revealed by high-throughput pyrosequencing of the 16S rRNA gene [J]. Vet Microbiol, 2013, 162(7)∶891-898
[3] Sibley CD, Peirano G, Church DL. Molecular methods for pathogen and microbial community detection and characterization: current and potential application in diagnostic microbiology [J]. Infect Genet Evol, 2012, 47(12)∶505-521
[4] Whitley R. The new age of molecular diagnostics for microbial agents [J]. New Engl J Med, 2008, 358(6)∶988-993
[5] Wang T, Cai G, Qiu Y, et al. Structural segregation of gut microbiota between colorectal cancer patients and healthy volunteers [J]. ISME J, 2011, 6(3)∶320-329
[6] Liu B, Faller LL, Klitgord N, et al. Deep sequencing of the oral microbiome reveals signatures of periodontal disease [J]. PLoS One, 2012, 7∶e37919
[7] Faveri M, Mayer MP, Feres M, et al. Microbiological diversity of generalized aggressive periodontitis by 16S rRNA clonal analysis [J]. Oral Microbiol Immun, 2008, 23(1)∶112-118
[8] Alcaraz LD, Belda-Ferre P, Cabrera-Rubio R, et al. Identifying a healthy oral microbiome through metagenomics [J]. Clin Microbiol Infec, 2012, 18(1)∶54-57
[9] Loesche WJ. Role of Streptococcus-Mutans in Human Dental Decay [J]. Microbiol Rev, 1986, 50(4)∶353-380
[10] Corby PM, Lyons-Weiler J, Bretz WA, et al. Microbial risk indicators of early childhood caries [J]. J Clin Microbiol, 2005, 43(18)∶5753-5759
[11] Costerton J, Stewart PS and Greenberg E. Bacterial biofilms: a common cause of persistent infections [J]. Science, 1999, 284(10)∶1318-1322
[12] 汪珍珍,王译彬,吴芳,等.健康儿童及成人唾液微生物多样性研究[J].口腔医学研究,2016,32(4)∶352-355

[1]	DONG Chen, HU Dawei. Analysis on Formation Mechanism of Oral Microbiome Diversity Induced by Delayed Response [J]. Journal of Oral Science Research, 2020, 36(11): 1016-1021.
[2]	GENG Shuangshuang, WANG Jian, YANG Li, FENG Xinxin, LU Lihua. Effect of Site Preservation on Periodontal Status of Periodontal Patients [J]. Journal of Oral Science Research, 2020, 36(11): 1022-1026.
[3]	WANG Junhui, WANG Zirui, ZHOU Zhifei, ZHANG Baize, GE Xin, CHEN Yujiang, DU Yang, WANG Xiaojing. Clinical Evaluation of Er:YAG Laser in Primary Caries Treatment [J]. Journal of Oral Science Research, 2020, 36(11): 1050-1054.
[4]	CAI Yilin, MENG Ziyan, WANG Chun, LIU Linhua, DONG Weili. Analysis of Clinical Epidemiological Data of 9942 Patients in Department of Periodontology [J]. Journal of Oral Science Research, 2019, 35(9): 850-853.
[5]	ZHOU Tian, KANG Na. Research Progress of Lactoferrin in Stomatology Medical Domain [J]. Journal of Oral Science Research, 2019, 35(8): 730-733.
[6]	ZHENG Jun-yuan, HE Li, HU Tu-qiang, WANG Mei-jun. Half-year Effect of Er-YAG in the Treatment of Periodontal Angular Bone Defect of Premolars in Elderly Patients: A Case Report [J]. Journal of Oral Science Research, 2019, 35(3): 307-308.
[7]	YU Ting, ZHAO Li, ZHANG Jin-cai, XUAN Dong-ying. Imbalanced Post-operative Weight Loss in Combined Obesity and Periodontitis Models [J]. Journal of Oral Science Research, 2019, 35(1): 23-27.
[8]	ZONG Ya-wen,WANG Fei-fei,LI Biao,LIU Bin. Research on the Relationship between Oral Pathogens and Alzheimer's Disease [J]. Journal of Oral Science Research, 2018, 34(6): 588-590.
[9]	YU Ting, ZHAO Li, ZHANG Jin-cai, XUAN Dong-ying. Serum Cytokine Response to Periodontal Infection in Overweight Mice [J]. Journal of Oral Science Research, 2018, 34(6): 644-647.
[10]	HOU Wen, SU Da, QUE Guo-ying. Correlation between Carbonic Anhydrase Ⅵ Level and Dental Caries among Children Aged 4-5 Years. [J]. Journal of Oral Science Research, 2018, 34(4): 363-366.
[11]	DONG Ying, CAO Hong-fei, YANG Ting, LI Bei-bei, ZHAO Jin. Distribution of Lactobacillus of the Uygur and Han Children in Bo Zhou, Xinjiang and Its Correlation with Caries [J]. Journal of Oral Science Research, 2018, 34(11): 1172-1176.
[12]	XU Jing-jing, TAO Ting-liang, LI Yu-hong.. Anti-caries Effect of A Bacteriophage Lysin. [J]. Journal of Oral Science Research, 2018, 34(10): 1052-1056.
[13]	LIU Yao, WANG Ling, YANG Wei-hong, HAN Lin-xiu, LIU Xing-rong.. Systematic Review on the Hall Technique for Children with Carious Primary Molars. [J]. Journal of Oral Science Research, 2018, 34(10): 1076-1080.
[14]	YE Zhen, GAO Kai-xian, YIN Xiao-qing. Prevalence of Dental Caries and Related Risk Factors in Primary School Students [J]. Journal of Oral Science Research, 2018, 34(1): 32-34.
[15]	LIU Yue-fang, ZHU Guang-xun. Research Progress of Music Therapy in Treatment of Periodontal Diseases [J]. Journal of Oral Science Research, 2018, 34(1): 100-102.