Effect of Anti-STAT3 shRNA on Dendritic Cell Maturation and Corresponding Targeted Anti-caries DNA Vaccine Construction.

doi:10.13701/j.cnki.kqyxyj.2016.03.006

Abstract

Abstract: Objective: To investigate the effect of anti-STAT3 shRNA on dendritic cell maturation and to construct an anti-careis DNA vaccine carrying anti-STAT3 shRNA to enhance the efficacy of DNA vaccination. Methods: Dendritic cell line DC2.4 was infected by lentivirus containing shRNA targeting mouse STAT3. Total cellular protein was collected to detect expression level of STAT3 by western blot. CD40, CD80 and CD86 of DC2.4 were analyzed by FACS. LPS was used after infection to stimulate DC maturation. Anti-careis DNA vaccine carrying anti-STAT3 shRNA was constructed by cloning shRNA fragment into DC target anti-caries DNA vaccine pGJA-P/VAX. The efficiency of its inhibition on STAT3 expression was confirmed in HEK-293 cells. Results: Expression of STAT3 decreased in DC2.4 infected by lentivirus containing shRNA targeting STAT3. The expression levels of CD40, CD80 and CD86 of DC2.4 increased upon STAT3 knockdown, which were further up-regulated after the stimulation of LPS. Anti-caries DNA vaccine with anti-STAT3 shRNA could inhibit STAT3 expression. Conclusion: Lentiviral mediated shRNA interference targeting STAT3 could inhibit the STAT3 expression level of DC2.4 and increase the expression of CD40, CD80 and CD86 molecules and the maturation of dendritic cells. Anti-caries DNA vaccine against STAT3 was constructed successfully.

Key words: STAT3 Lentivirus, Dendritic cell, shRNA, DNA vaccine

CLC Number:

R780.1

LIU Miao-miao, ZHANG Yan, ZHANG Si, FAN Ming-wen, GUO Ji-hua. Effect of Anti-STAT3 shRNA on Dendritic Cell Maturation and Corresponding Targeted Anti-caries DNA Vaccine Construction.[J]. Journal of Oral Science Research, 2016, 32(3): 234-238.

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