Journal of Oral Science Research

Journal of Oral Science Research ›› 2018, Vol. 34 ›› Issue (3): 249-253.DOI: 10.13701/j.cnki.kqyxyj.2018.03.009

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Effects of Hydrostatic Pressure on Mechanical Property of Tissue Engineering Cartilage Constructed by BMSCs/PRF Compound Membranes.

ZHAO Ying1, CHEN Hui2, ZHANG Min1*   

  1. 1. State Key Laboratory of Military Stomatology, National Center for Oral Diseases and Clinical Medicine, Department of General Dentistry & Emergency, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, China;
    2. Tongren Hospital of WuHan University, Wuhan 430060, China
  • Received:2018-01-24 Online:2018-03-30 Published:2018-03-27

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Abstract: Objective: To evaluate the effects of hydrostatic pressure on mechanical property of tissue engineering cartilage which was constructed by BMSCs/PRF compound membranes. Methods: BMSCs were obtained by density gradient centrifugation. BMSCs cell sheet was obtained after cell surface markers detection. After PRF membrane was obtained by whole blood centrifuge, BMSCs/PRF compound membranes were constructed. BMSCs/PRF constructs were randomized into 3 groups. BMSCs/PRF construct cultured without any mechanical stimulation or chemical induction was adopted as control group. BMSCs/PRF compound membranes treated with chondrogenic medium was induction group. BMSCs/PRF compound membranes treated with chondrogenic medium and hydrostatic pressure was defined as pressure+ induction group. Gene expression of Sox-9, aggrecan, and Col-II were analyzed by real-time PCR for all groups. HE staining and toluidine blue staining were performed to observe the histomorphology. Elasticity moduli of BMSCs/PRF compound membranes of each group were measured by ElectroForce System3200. Results: The mRNA expressions of Sox-9, aggrecan, and Col-II in induction group and pressure+ induction group were significantly higher than those of control group. Besides, the expressions of aggrecan and Col-II in pressure+induction group were significantly higher than those of the induction group. HE staining showed that the density population of the chondrocytes in pressure+induction group increased greatly compared with control and induction group. The metachromatic particles could be found by toluidine blue staining in pressure+induction group. Elasticity moduli of tissue engineering cartilage constructed by BMSCs/PRF compound membranes inpressure+induction group was also enhanced compared with that of control and induction group. Conclusion: Hydrostatic pressure promotes the quality and mechanical property of tissue engineering cartilage constructed by BMSCs/PRF compound membranes.

Key words: Hydrostatic pressure, BMSCs, PRF, Chondrogenesis, Elasticity moduli