Reconstruction of Condylar Bone and Expressions of Sox9, MMP-13, and VEGF following Rabbit Articular Disc Perforation

doi:10.13701/j.cnki.kqyxyj.2019.10.020

Abstract

Abstract: Objective: To analyze the reconstruction of subchondral bone of TMJOA, to investigate the expression of Sox9, MMP-13, and VEGF, and to explore the mechanism of their effects on subchondral bone reconstruction. Methods: A full-thickness perforation in the diameter of 2.5 mm was made on the posterolateral side of the articular disc. BV/TV, BS/BV, Tb.Th, Tb.Sp, and Tb.N of subchondral bone were calculated by micro-CT. HE staining and immunohistochemical staining was carried out to analyze the expression and localization of Sox9, MMP-13, and VEGF, and semi-quantitative analysis was carried out. Results: Experimental group (EG) showed typical histopathological features of OA. BV/TV, Tb.Th, and Tb.N of EG were significantly decreased (P＜0.05), and BS/BV and Tb.Sp of EG were significantly increased (P＜0.05). The expression of Sox9, MMP-13, and VEGF in the subchondral bone of EG were significantly higher than those of sham operation group (SG) (P＜0.05). Conclusion: The reconstruction of subchondral bone is an important feature of TMJOA. Sox9, MMP-13, and VEGF play an important role in bone remodeling.

Key words: temporomandibular joint, osteoarthritis, perforation of articular disc, subchondral bone

XIAO Yanju, WU Guomin, ZHANG Yaoyao, CHEN Kai, WANG Xiangshen. Reconstruction of Condylar Bone and Expressions of Sox9, MMP-13, and VEGF following Rabbit Articular Disc Perforation[J]. Journal of Oral Science Research, 2019, 35(10): 994-1000.

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