Effect of PMX205 on Osteoclastic Differentiation of RAW264.7 Cells induced by Anaphylatoxin C5a

doi:10.13701/j.cnki.kqyxyj.2021.07.013

Abstract

Abstract: Objective: To investigate the effect of PMX205 on osteoclastic differentiation of RAW264.7 cells induced by anaphylatoxin C5a. Methods: The viability of RAW264.7 treated by PMX205 was detected with MTT assays, and then RAW264.7 were divided into three groups: control group; C5a group, in which cells were induced osteoclastogenic differentiation in medium supplemented with 10 ng/mL RANKL and 1 μmol/L C5a; C5a+PMX205 group, in which cells were treated with 1 μg/mL PMX205, 10 ng/mL RANKL, and 1 μmol/L C5a. The differentiation of RAW264.7 to osteoclast-like cells was quantified by tartrate-resistant acid phosphatase (TRAP) staining, and genes expression associated with osteoclastic differentiation were assessed by real-time quantitative polymerase chain reaction (qRT-PCR). Results: PMX205 significantly inhibited the osteoclastic differentiation induced by C5a in RAW264.7 cells without cytotoxicity. The inhibitory effects of PMX205 on differentiation-related signaling molecules resulted in significant repression of c-fos, TRAF6, and NFATc1 expression, compared with the C5a group (P<0.01), which was still higher than that of control group (P<0.01). Conclusion: PMX205 decreased osteoclastic differentiation of RAW264.7 induced by C5a through C5a-C5aR axis.

Key words: periodontitis, C5a, RAW264.7, PMX205, Osteoclast

JIN Ting, CUI Leihua, WANG Huining, HOU Yubo. Effect of PMX205 on Osteoclastic Differentiation of RAW264.7 Cells induced by Anaphylatoxin C5a[J]. Journal of Oral Science Research, 2021, 37(7): 637-640.

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