Copper-doped Titanium Alloy Inhibited RANKL-induced Osteoclasts Differentiation in Vitro

doi:10.13701/j.cnki.kqyxyj.2021.04.020

Abstract

Abstract: Objective: To study the effect of copper-doped titanium alloys on osteoclasts differentiation. Methods: Copper-doped titanium alloys were prepared by using the technique of selective laser melting (SLM). Mouse monocytes (RAW264.7) were induced to differentiate to osteoclasts by RANKL. Copper-doped titanium alloys was co-cultured with osteoclasts. The morphology of osteoclasts was observed under a fluorescence microscope. The number of osteoclasts was recorded, and the expression of genes related to osteoclast differentiation was detected by real-time fluorescence quantitative PCR. Results: Induced by RANKL, mouse monocytes could differentiate into osteoclasts in vitro. Copper-doped titanium alloys could not only inhibit the differentiation of monocytes (RAW264.7) which generated osteoclasts, but also inhibit the expression of osteoclast-related genes CTSK, TRAP, NFATc1, and NF-κB. Conclusion: Copper-doped titanium alloys can inhibit the activity of osteoclasts and is expected to be used in the preparation of titanium mesh which has potential application value for alveolar bone regeneration.

Key words: titanium alloy, copper, osteoclast differentiation, scaffold

XU Xiongcheng, ZHUO Jin, XU Yanmei , LUO Kai, CHEN Chao, ZHONG Quan. Copper-doped Titanium Alloy Inhibited RANKL-induced Osteoclasts Differentiation in Vitro[J]. Journal of Oral Science Research, 2021, 37(4): 371-374.

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