Par3 Has an Effect on Cellular Junction and Biological Behavior of OLCs

doi:10.13701/j.cnki.kqyxyj.2022.04.012

Abstract

Abstract: Objective: To investigate the effect of partitioning defective-3 (Par3) on cell junction and biological behavior of odontoblast cells (OLCs) in vitro. Methods: The expression and distribution of Par3 in vivo and in vitro were examined by immunofluorescence (IF). Western blotting was used to assess the expression level of Par3 in OLCs at inflammatory and physiological state. Par3 siRNA was transfected in OLCs to interfere the expression of Par3. The continuity and structure of the intercellular junction were observed by transmission electronic microscopy (TEM). Western blotting and IF were performed to investigate the expression and distribution of cell junction related protein ZO-1. 5-Ethynyl-2'-deoxyuridine (EdU), transwell assay, scratch-wound assay, and flow cytometry were used to assess cell biological behavior like proliferation, migration, and apoptosis. Results: In rat pulp tissue, Par3 located along the odontoblast layer. Par3 located at cytoplasm in OLCs. The expression of Par3 might be down-regulated at inflammatory state. The cellular junction between OLCs was discontinuous and the expression of total ZO-1 or ZO-1 located at membrane in OLCs was reduced after eliminated Par3. The growth rate and the number of migrated cells in siPar3 group were increased. While the apoptosis rate was decreased in siPar3 group compared with the control group. Conclusion: Downregulation of Par3 can disrupt the intercellular junction of OLCs by regulating the expression and distribution of ZO-1, while the capacity of cellular proliferation and migration is enhanced.

Key words: odontoblast cell, cell junction, Par3, reaction dentin

WANG Jueyu, XAIO Min, BAI Yu, CHENG Xiaogang, WU Haoze, KUANG Jinxin, YU Qing. Par3 Has an Effect on Cellular Junction and Biological Behavior of OLCs[J]. Journal of Oral Science Research, 2022, 38(4): 350-356.

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