Establishment and Identification of Human Tongue Squamous Cell Carcinoma Cell Line WU-TSC-1

doi:10.13701/j.cnki.kqyxyj.2022.04.013

Abstract

Abstract: Objective: To establish a human tongue squamous cell carcinoma (TSCC) cell line WU-TSC-1 and to identify its basic biological characteristics. Methods: The excised tissue from a patient with TSCC was primary cultured. After passage, the TSCC cell line WU-TSC-1 was established. Its morphology and growth characteristics were observed and recorded through microscope. Short tandem repeat analysis (STR) of genomic DNA on this cell was conducted. Its doubling time, agglutination reaction, cell surface markers, chromosome karyotype, and tumorigenic ability in nude mice were also examined. Results: WU-TSC-1 cells had been subcultured for more than 50 passages which were confirmed to be human-derived tumor cells with no other cell contamination. In vitro, the cells lost contact inhibition and had a uniform oval or squares shape and non-diploid chromosomes. Its doubling time was calculated as 51.15 h. In vivo, the cell line showed tumorigenesis ability. Conclusion: This study has successfully established a human TSCC cell line WU-TSC-1 which provided a new cell line for revealing the pathogenesis of TSCC as well as exploring useful diagnostic indicators and treatment options.

Key words: tongue squamous cell carcinoma, cell line, primary culture

ZHENG Xiaofeng, SUN Yanan, HU Yaying, LV Yinan, YANG Ke, LI Yiwei, ZHANG Jiali. Establishment and Identification of Human Tongue Squamous Cell Carcinoma Cell Line WU-TSC-1[J]. Journal of Oral Science Research, 2022, 38(4): 357-361.

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