Effect of CGRP on Mock-microenvironment around Implants and the Building-up of CGRP Sustained Release System on Titanium Plate Surface

doi:10.13701/j.cnki.kqyxyj.2023.06.010

Abstract

Abstract: Objective: To explore the effect of calcitonin gene related peptide (CGRP) on human bone marrow mesenchymal stem cells (hBMSCs) and its optimal concentration, and to build up a CGRP sustained-release system on the surface of titanium plate to detect its nerve growth promoting effect. Methods: The proliferation and differentiation of hBMSCs induced by different concentrations of CGRP (0-10^-6 mol/L) were measured. According to the concentration of CGRP induced the best cell activity of hBMSCs, the effects of CGRP on the activity of alkaline phosphatase and the expression of osteogenic related genes and proteins were determined. The surface of titanium plate was modified by dopamine chelating CGRP compound. The sustained release effect of CGRP was checked by Elisa in 28 days, and its effect on human neuroblastoma cells (SH-SY5Y) was also measured. Results: CGRP with a concentration of 10^-7 mol/L had the effect of promoting osteogenic differentiation of hBMSCs and neuronal differentiation of SH-SY5Y in vitro. Dopamine chelating CGRP composite modified titanium plate had a sustained release effect and promoted the proliferation and differentiation of nerve cells.Conclusion: Dopamine chelating CGRP composite modified titanium plate surface might build up a way to apply CGRP in the microenvironments round implants, forming a new strategy to promote the osseointegration and osseoperception of dental implants.

Key words: calcitonin gene related peptide, sustained-release system, implant, osseointegration, peri-implant innervation

XUE Shenghao, WU Meichen, LI Jian, LEI Lei, LIU Zhongning, JIANG Ting. Effect of CGRP on Mock-microenvironment around Implants and the Building-up of CGRP Sustained Release System on Titanium Plate Surface[J]. Journal of Oral Science Research, 2023, 39(6): 515-521.

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