EGCG Crosslinked Biomimetic Mineralized Decellularized Matrix of Filefish Skin As A Membrane for Guided Bone Tissue Regeneration

doi:10.13701/j.cnki.kqyxyj.2024.05.013

Abstract

Abstract: Objective: To construct decellularized matrix of filefish skin and crosslink it to be biomimetic mineralization template as a guided bone regeneration membrane. Methods: Filefish skin of marine origin was selected, and the decellularized matrix of filefish skin (filefish skin decellularized matrix,FS-ECM) was prepared by a combined physicochemical method. The structural features of FS-ECM were preliminarily explored by HE staining, scanning electron microscope (SEM), X-ray diffraction (XRD), and water contact angle. Then, the cross-linking modification of epigallocatechin gallate (EGCG) was utilized (EGCG crosslinked filefish skin decellularized matrix,E-FS-ECM) to improve the material's anti-enzymatic and mechanical properties. The EGCG-collagen in vitro biomimetic mineralization template (EGCG crosslinked biomimetic mineralized filefish skin decellularized matrix, EB-FS-ECM) was constructed through the modification of EGCG, and the collagen biomimetic mineralization strategy was used to further improve the various properties. The differences in the physicochemical properties of the materials before and after modification were evaluated using SEM, mapping, water contact angle, elastic modulus, thermogravimetric analysis, and in vitro degradation. Results: There was no significant difference between the surface structural properties of FS-ECM at different sites (P>0.05), and FS-ECM contained a certain amount of hydroxyapatite crystals on the outer surface. After the modification by EGCG cross-linking and construction of biomimetic mineralization templates, the hydrophilicity, mechanical strength, thermal stability, and resistance to enzymatic degradation of E-FS-ECM and EB-FS-ECM were significantly higher than those of FS-ECM (P<0.05). Conclusion: EGCG cross-linking and biomimetic mineralization significantly improved the hydrophilicity, thermal stability, and mechanical strength, and slowed down the degradation rate of FS-ECM. Decellularized matrix of filefish skin through modification is expected to be an suitable material for GBR membranes.

Key words: filefish skin, decellularization, EGCG, cross-linking, biomimetic mineralized

SHEN Shengjie, SUN Ning, XIAO Ting, LI Quanli. EGCG Crosslinked Biomimetic Mineralized Decellularized Matrix of Filefish Skin As A Membrane for Guided Bone Tissue Regeneration[J]. Journal of Oral Science Research, 2024, 40(5): 448-455.

References

[1] Dimitriou R, Mataliotakis GI, Calori GM, et al. The role of barrier membranes for guided bone regeneration and restoration of large bone defects: current experimental and clinical evidence [J]. BMC Med, 2012, 10:81.
[2] Ren Y, Fan L, Alkildani S, et al. Barrier membranes for guided bone regeneration (GBR): A focus on recent advances in collagen membranes [J]. Int J Mol Sci, 2022, 23(23):14987.
[3] Kim K, Su Y, Kucine AJ, et al. Guided bone regeneration using barrier membrane in dental applications [J]. ACS Biomater Sci Eng, 2023, 9(10):5457-5478.
[4] Sbricoli L, Guazzo R, Annunziata M, et al. Selection of collagen membranes for bone regeneration: A literature review [J]. Materials (Basel), 2020, 13(3):786.
[5] de Souza A, de Almeida Cruz M, de Araújo TAT, et al. Fish collagen for skin wound healing: a systematic review in experimental animal studies [J]. Cell Tissue Res, 2022, 388(3):489-502.
[6] Pati F, Datta P, Adhikari B, et al. Collagen scaffolds derived from fresh water fish origin and their biocompatibility [J]. J Biomed Mater Res A, 2012, 100(4):1068-1079.
[7] Liu S, Lau CS, Liang K, et al. Marine collagen scaffolds in tissue engineering [J]. Curr Opin Biotechnol, 2022, 74:92-103.
[8] Cho JK, Jin YG, Rha SJ, et al. Biochemical characteristics of four marine fish skins in Korea [J]. Food Chem, 2014, 159:200-207.
[9] Oslan SNH, Li CX, Shapawi R, et al. Extraction and characterization of bioactive fish by-product collagen as promising for potential wound healing agent in pharmaceutical applications: Current trend and future perspective [J]. Int J Food Sci, 2022, 2022:9437878.
[10] Subhan F, Ikram M, Shehzad A, et al. Marine collagen: An emerging player in biomedical applications [J]. J Food Sci Technol, 2015, 52(8):4703-4707.
[11] Silva TH, Moreira-Silva J, Marques AL, et al. Marine origin collagens and its potential applications [J]. Mar Drugs, 2014, 12(12):5881-5901.
[12] Subhan F, Hussain Z, Tauseef I, et al. A review on recent advances and applications of fish collagen [J]. Crit Rev Food Sci Nutr, 2021, 61(6):1027-1037.
[13] Gao Y, Wang S, Shi B, et al. Advances in modification methods based on biodegradable membranes in guided bone/tissue regeneration: A review [J]. Polymers (Basel), 2022, 14(5):871.
[14] Oryan A, Kamali A, Moshiri A, et al. Chemical crosslinking of biopolymeric scaffolds: Current knowledge and future directions of crosslinked engineered bone scaffolds [J]. Int J Biol Macromol, 2018, 107(Pt A):678-688.
[15] Chu C, Deng J, Xiang L, et al. Evaluation of epigallocatechin-3-gallate (EGCG) cross-linked collagen membranes and concerns on osteoblasts [J]. Mater Sci Eng C Mater Biol Appl, 2016, 67:386-394.
[16] Jackson JK, Zhao J, Wong W, et al. The inhibition of collagenase induced degradation of collagen by the galloyl-containing polyphenols tannic acid, epigallocatechin gallate and epicatechin gallate [J]. J Mater Sci Mater Med, 2010, 21(5):1435-1443.
[17] Islam MT, Felfel RM, Abou Neel EA, et al. Bioactive calcium phosphate-based glasses and ceramics and their biomedical applications: A review [J]. J Tissue Eng, 2017, 8:2041731417719170.
[18] Sam G, Pillai BR. Evolution of barrier membranes in periodontal regeneration-"Are the third generation membranes really here?" [J]. J Clin Diagn Res, 2014, 8(12):ZE14-ZE17.
[19] Wang Y, Van Manh N, Wang H, et al. Synergistic intrafibrillar/extrafibrillar mineralization of collagen scaffolds based on a biomimetic strategy to promote the regeneration of bone defects [J]. Int J Nanomedicine, 2016, 11:2053-2067.
[20] 楚水晶,农绍庄,柳春山,等.酶法提取马面鱼鱼皮胶原蛋白的工艺研究[J].食品科技,2010,35(5):234-237+241.
[21] Zeng A, Li H, Liu J, et al. The progress of decellularized scaffold in stomatology [J]. Tissue Eng Regen Med, 2022, 19(3):451-461.
[22] Wu L, Shao H, Fang Z, et al. Mechanism and effects of polyphenol derivatives for modifying collagen [J]. ACS Biomater Sci Eng, 2019, 5(9):4272-4284.
[23] Wu L, Wang Q, Li Y, et al. A dopamine acrylamide molecule for promoting collagen biomimetic mineralization and regulating crystal growth direction [J]. ACS Appl Mater Interfaces, 2021, 13(33):39142-39156.
[24] Elgali I, Omar O, Dahlin C, et al. Guided bone regeneration: materials and biological mechanisms revisited [J]. Eur J Oral Sci, 2017, 125(5):315-337.
[25] Yang Z, Wu C, Shi H, et al. Advances in barrier membranes for guided bone regeneration techniques [J]. Front Bioeng Biotechnol, 2022, 10:921576.
[26] León-López A, Morales-Peñaloza A, Martínez-Juárez VM, et al. Hydrolyzed collagen-sources and applications [J]. Molecules, 2019, 24(22):4031.
[27] Song WK, Liu D, Sun LL, et al. Physicochemical and biocompatibility properties of type Ⅰ collagen from the skin of nile tilapia (oreochromis niloticus) for biomedical applications [J]. Mar Drugs, 2019, 17(3):137.
[28] Sinthusamran S, Benjakul S, Kishimura H. Comparative study on molecular characteristics of acid soluble collagens from skin and swim bladder of seabass (Lates calcarifer) [J]. Food Chem, 2013, 138(4):2435-2441.
[29] Ge B, Wang H, Li J, et al. Comprehensive assessment of nile tilapia skin (oreochromis niloticus) collagen hydrogels for wound dressings [J]. Mar Drugs, 2020, 18(4):178.
[30] Zhang X, Chen X, Hong H, et al. Decellularized extracellular matrix scaffolds: Recent trends and emerging strategies in tissue engineering [J]. Bioact Mater, 2021, 10:15-31.
[31] Abaricia JO, Shah AH, Musselman RM, et al. Hydrophilic titanium surfaces reduce neutrophil inflammatory response and NETosis [J]. Biomater Sci, 2020, 8(8):2289-2299.
[32] Lang NP, Salvi GE, Huynh-Ba G, et al. Early osseointegration to hydrophilic and hydrophobic implant surfaces in humans [J]. Clin Oral Implants Res, 2011, 22(4):349-356.
[33] Gou M, Huang YZ, Hu JG, et al. Epigallocatechin-3-gallate cross-linked small intestinal submucosa for guided bone regeneration [J]. ACS Biomater Sci Eng, 2019, 5(10):5024-5035.