Study on Reconstruction of Bone Defects by 3D-Printed HA-SA-CMCS Three-Dimensional Scaffold Combined with Masquelet Technique

doi:10.13701/j.cnki.kqyxyj.2026.06.008

Abstract

Abstract: Objective: To develope a hydroxyapatite/sodium alginate/carboxymethyl chitosan (HA-SA-CMCS, referred to as HSC) three-dimensional scaffold with favorable blood supply potential by integrating tissue engineering principles with the Masquelet technique, and evaluate its bone repair efficacy. Methods: HSC three-dimensional scaffolds were fabricated using 3D printing. The mechanical properties, in vitro degradability, and mineralization capacity of the scaffolds were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), a universal testing machine, and other techniques. The in vitro biocompatibility and osteogenic potential were assessed through cell culture with rabbit bone marrow mesenchymal stem cells (BMSCs), including alkaline phosphatase (ALP) and alizarin red S (ARS) staining. Finally, the in vivo osteogenic performance was evaluated using a skull defect model in New Zealand white rabbits. Results: The HSC scaffold exhibited excellent mechanical properties, hydrophilicity, and biocompatibility. ALP and ARS staining demonstrated that the HSC scaffold significantly promoted the osteogenic differentiation of rabbit BMSCs. Animal experiments further indicated that the HSC scaffold group had superior bone repair performance compared to the blank control group. Conclusion: The 3D-printed HSC three-dimensional scaffold combined with the Masquelet technique can effectively promote bone tissue regeneration, accelerate the repair of rabbit skull defects, and open up a highly promising new approach for the treatment strategy of bone defects.

Key words: 3D printing, bone defect, masquelet technique, bone tissue engineering

WANG Jianzhe, ZHU Xiaojing, CHEN Lingling, SHI Zhenyu, WANG Yibing, FENG Cheng, LI Qiong, DU Qihao, WU Ye. Study on Reconstruction of Bone Defects by 3D-Printed HA-SA-CMCS Three-Dimensional Scaffold Combined with Masquelet Technique[J]. Journal of Oral Science Research, 2026, 42(6): 504-511.

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