Effect of LP-17 on Porphyromonasgingivalis Lipopolysaccharide Caused Periodontitis.

doi:10.13701/j.cnki.kqyxyj.2018.03.027

Abstract

Abstract: Objective: To explore the effect of LP-17, an inhibitor of triggering receptors expressed on myeloid cells-1 (TREM-1) on porphyromonasgingivalis lipopolysaccharide (P. gingivalis-LPS) induced periodontitis in mice. Methods: P. gingivalis-LPS was injected into maxillary second molar sulcus of C57BL/6 mice to construct experimental periodontitis model. Mice were treated with LP-17 peptides by intraperitoneal injection every day, and PBS was set as the control. After 14 days, all mice were sacrificed. Micro-CT was used to measure the alveolar bone absorption. HE staining was used to assess pathological alteration and TRAP staining was used to detect osteoclasts in periodontium. In addition, the expression of TREM-1 was determined by immunohistochemistry. Results: P. gingivalis-LPS induced experimental periodontitis in mice. LP-17 reduced the degree of collagen fibrosis and inflammatory cell infiltration. Additionally, LP-17 inhibited the expression of TREM-1 in periodontal tissue. Furthermore, LP-17 treatment notably decreased the number of osteoclasts and alveolar bone absorption. Conclusion: LP-17 peptides alleviated the inflammatory response in periodontal tissue, thus arrested the progress of periodontitis.

Key words: TREM-1, LP-17, Periodontitis

KE Xiao-jing, LEI Lang, YAN Fu-hua. Effect of LP-17 on Porphyromonasgingivalis Lipopolysaccharide Caused Periodontitis.[J]. Journal of Oral Science Research, 2018, 34(3): 323-326.

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