Gene Expression, Purification, and Activity Determination of Alanine Racemase from Streptococcus Mutans

doi:10.13701/j.cnki.kqyxyj.2018.06.006

Abstract

Abstract: Objective: To purify and measure activity of alanine racemase from Streptococcus mutans (S. mutans). Methods: In the study, expression of recombinant pET-smu.1834c was induced by IPTG, and the protein was purified using Ni-NTA resin with eluotropic buffers. Results: The protein encoded by smu.1834c was successfully purified and it was found that the protein could catalyze the conversion of L-Ala into D-Ala in vitro. Conclusion: D-cycloserine (DCS) was able to inhibit the activity of purified Alanine racemase (Alr) in a dose-dependent manner. This study confirmed that smu.1834c was encoded Alr in S. mutans. DCS can inhibit the activity of Alr.

Key words: Streptococcus mutans, Alanine racemase, D-alanine, D-cycloserine

GUO Xiao, QIU Wei, ZHOU Xue-dong, CHENG Lei, LI Ming-yun. Gene Expression, Purification, and Activity Determination of Alanine Racemase from Streptococcus Mutans[J]. Journal of Oral Science Research, 2018, 34(6): 597-600.

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