Protective Effects of Hyaluronic Acid on Synovial Fibroblasts from Temporomandibular Joint Against Oxidative Stress Induced by Hydrogen Peroxide

doi:10.13701/j.cnki.kqyxyj.2019.12.010

Abstract

Abstract: Objective: To observe the effects of oxygen free radicals on the synovial fibroblasts from the temporomandibular joint and the protective effects of hyaluronic acid against the oxidative stress. Methods: Synovial tissues were harvested from the rabbit temporomandibular joint and cultured en bloc. The synovial fibroblasts gained at 3-8 passages were used for further study. Hydrogen peroxide was introduced to mimic the effect of oxygen free radicals within the temporomandibular joint, and the hyaluronic acid was added to counteract the effects of reactive oxygen species. Results: There was no difference in the vitality of synovial fibroblasts between H₂O₂ groups (5 μmol/L and 10 μmol/L) and control group (P＞0.05),while the 100 μmol/L H₂O₂ overinhibited the viability of the synovial fibroblasts. 50μmol/L H₂O₂ appropriately inhibited the activity of synovial fibroblasts (60%) and was selected as the ideal stimulus. In the serial concentrations of hyaluronic acid, the 0.01% and 0.05% groups significantly recovered the activity of synovial fibroblasts in contrast to the low or high concentrations of HA (P＜0.05). Conclusion: Proper oxidative stress to the synovial fibroblasts can be induced by 50 μmol/L H₂O₂, while the 0.01% and 0.05% HA can protect the synovial fibroblasts from the damage of oxygen free radicals.

Key words: temporomandibular joint, synovial fibroblast, oxidative stress, hyaluronic acid, cell culture

CAI Hengxing, LI Yingjie, LONG Xing. Protective Effects of Hyaluronic Acid on Synovial Fibroblasts from Temporomandibular Joint Against Oxidative Stress Induced by Hydrogen Peroxide[J]. Journal of Oral Science Research, 2019, 35(12): 1141-1144.

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