Expression and Significance of EpCAM and β-catenin in Mucoepidermoid Carcinoma of Salivary Glands

doi:10.13701/j.cnki.kqyxyj.2020.05.008

Abstract

Abstract: Objective: To investigate the expression and clinicopathologic significance of EpCAM and β-catenin in mucoepidermoid carcinoma (MEC). Methods: The expressions of EpCAM and β-catenin in 57 cases of MEC and 20 cases of normal salivary gland tissues were detected by immunohistochemical staining (Envision method). Results: The positive expression rate of EpCAM and β-catenin in MEC was significantly higher than that in normal tissues (P=0.002, P=0.001). The positive expression rates of EpCAM in MEC with high, medium, and low differentiation were 36.36%, 61.11%, and 82.35%, respectively (P=0.015), and the positive expression rates of β-catenin were 45.45%, 72.22%, and 88.24%, respectively, with statistically significant differences (P=0.016). Both EpCAM and β-catenin expression were correlated with patient age (P=0.004, P=0.004). EpCAM was correlated with lymph node metastasis (P=0.024), and the expression of EpCAM and β-catenin was unrelated to the gender of patients and the location of tumor. The expression of EpCAM and β-catenin in MEC was positively correlated (R=0.48, P < 0.001). Conclusion: Expressions of EpCAM and β-catenin were associated with MEC malignancies. Joint tests could help to evaluate MEC, assist in predicting prognosis, and provide basis for the targeted treatment of MEC.

Key words: mucoepidermoid carcinoma, EpCAM, β-catenin

ZHU Yunna, ZHENG Huixia, XIAO Hong, WAN Huili, LV Jiale, LIANG Gang, LI Ning. Expression and Significance of EpCAM and β-catenin in Mucoepidermoid Carcinoma of Salivary Glands[J]. Journal of Oral Science Research, 2020, 36(5): 433-436.

References

[1] 赵志国,高丹,王瑾,等.896例腮腺肿瘤临床回顾分析[J].上海口腔医学,2017,26(6):605-609.
[2] Brandwein MS,Ivanov K,Wallace DI,et al. Mucoepidermoid carci-noma:a clinicopathologic study of 80 patients with special reference to histological grading[J].Am J Surg Pathol,2001,25(7):835-845.
[3] 许宁,朱恩新,刘婷姣,等.CyclinD1和ki-67与涎腺黏液表皮样癌发生及预后的相关性研究[J].口腔医学研究,2008,24(6):625-628.
[4] 毛建英,冯建中,盛陈卓娅,等.上皮细胞黏附分子在非小细胞肺癌组织中表达及其临床意义[J].中华外科学杂志,2016,33(11):2576-2579.
[5] Kroepil F, Dulian A, Vallbohmer D, et al. High EpCAM expression is linked to proliferation and Lauren clas Sification in gastric cancer [J]. BMC Res Notes, 2013,6:253.
[6] Kamal NM, Salem HM, Dahmoush HM, et al. Immunohistochemical expression of epithelial cell adhesion molecule(EpCAM) in mucoepidermoid carcinoma compared to normal salivary gland tissues[J].Arch Oral Biol,2017,79: 87-94.
[7] Ralhan R, Cao J, Lim T, et al. EpCAM nuclear localization identifies aggressive thyroid cancer and is a marker for poor prognosis[J]. BMC Cancer, 2010, 10:331.
[8] Kuan II, Lee CC, Chen CH, et al.The extracellular domain of epithelial cell adhesion molecule (EpCAM) enhances multipotency of mesenchymal stem cells through EGFR-LIN28-LET7 signaling[J]. J Biol Chem, 2019, 294(19):7769-7786.
[9] Liang KH, Tso HC, Hung SH, et al. Extracellular domain of EpCAM enhances tumor progression through EGFR signaling in colon cancer cells[J].Cancer Lett, 2018, 433:165-175.
[10] Chen W, Zhang YW, Li Y, et al. Constitutive expression of Wnt／β-catenin target genes promotes proliferation and invasion of liver cancer stem cells[J]. Mol Med Rep,2016,13(4):3466-3474.
[11] Jang GB,Hong IS,Kim RJ,et al. Wnt/β-eatenin small.Molecule inhibitor CWP232228 preferentially inhibits the growth of breast cancer stem-like cells[J].Cancer Res, 2015, 75(8):1691-1702.
[12] Singh G, Rumende CM, Amin Z. Thymoma: diagnosis and treatment [J]. Acta Med Indones, 2011, 43(1): 74-78.
[13] Doucas H, Garcea G, Neal CP, et al. Distinct changes in the Wnt signalling pathway in gastrointestinal cancers and their prognostic significance [J]. Eur J Cancer, 2005, 41(3):365-379.
[14] 金雯,陈顺华,尹玉,等.β-catenin不同表达模式与结直肠癌临床病理因素的关系[J].临床与实验病理学杂志,2017,33(6):596-600.
[15] Yu T, Ma Y, Wang H, et al. EpCAM intracellular domain promotes porcine cell reprogramming by upregulation of pluripotent gene expression via beta-catenin signaling[J]. Sci Rep, 2017, 7:46315.
[16] Yamashita T,Budhu A,Forgues M,et al. Activation of hepatic stemcell marker EpCAM by Wnt-beta-catenin signaling in hepatocellular carcinoma[J]. Cancer Res, 2007, 67(22):10831-10839.
[17] Cioffi M,Dorado J,Baeuerle PA,et al. EpCAM/CD3-Bispecific Tcell engaging antibody MT110 eliminates primary human pancreatic cancer stem cells [J]. Clin Cancer Res,2012,18(2) : 465-474.
[18] Ströhlein MA, Lordick F, Rüttinger D, et al.Immunotherapy of peritoneal carcinomatosis with the antibody catumaxomab in colon, gastric, or pancreatic cancer: an open-label, multicenter phase Ⅰ/Ⅱ trial [J]. Onkologie, 2011, 34(3):101-108.