Expression and Prognostic Significance of microRNAs in Peripheral Blood of Patients with Oral Squamous Cell Carcinoma

doi:10.13701/j.cnki.kqyxyj.2020.05.007

Abstract

Abstract: Objective: To screen differentially expressed microRNAs (miRNAs) in peripheral blood of patients with oral squamous cell carcinoma (OSCC) by qRT-PCR, and further to explore the relationship between differentially expressed miRNAs and clinical parameters and prognosis. Methods: The changes of miRNA in peripheral blood of patients with OSCC were observed by qRT-PCR, and the relationship between miRNA and clinicopathological parameters and prognosis was analyzed. Results: A total of 84 kinds of miRNAs were detected, of which 8 were up-regulated more than twice in peripheral blood of patients with OSCC and 18 were down-regulated less than 0.5 times. The expression of miR-222-3p and miR-423-5p in peripheral blood was significantly correlated with pTNM stage and lymph node metastasis (P＜0.05). The patients with high expression of miR-222-3p and low expression of miR-423-5p had a worse prognosis (P＜0.05). Multivariate analysis showed that the overexpression of miR-222-3p was an independent prognostic factor for the OSCC patients. Conclusions: miR-222-3p and miR-423-5p were abnormally expressed in the peripheral blood in OSCC patients, and they were expected to be biomarkers for early diagnosis and predicting prognosis of OSCC.

Key words: OSCC, miR-222-3p, miR-423-5p, biomarkers, prognosis

YANG Kaicheng, YANG Lei, ZHAO Jianguang, LUO Fengyu, CHEN Yanping, CUI Zifeng, CHEN He, MAN Shasha. Expression and Prognostic Significance of microRNAs in Peripheral Blood of Patients with Oral Squamous Cell Carcinoma[J]. Journal of Oral Science Research, 2020, 36(5): 428-432.

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