Differential Expression of Extracellular Matrix Protein DMP1-C and DMP1-N in Condylar Cartilage Before and After Occlusal Trauma Treatment

doi:10.13701/j.cnki.kqyxyj.2020.05.010

Abstract

Abstract: Objective: To analyze the molecular mechanism of occlusal trauma on condylar mineralization in rats and to determine the clinical significance of occlusal adjustment through observing the histological changes of rats condylar cartilage and subchondral trabeculae and the differential expression of ECM protein DMP1-C and DMP1-N terminal in condylar cartilage. Methods: The rat models with 2 weeks occlusal trauma, 2 weeks after removal of 2 weeks occlusal trauma, and 4 weeks after removal of 2 weeks occlusal trauma. HE staining was used to observe the histological changes of condylar cartilage and subchondral trabeculae among the anterior, middle, and posterior of the condylar process, and immunohistochemistry was used to observe the differential expression of ECM protein DMP1-C and DMP1-N among the anterior, middle, and posterior of the condylar process before and after traumatic treatment. Results: Compared with the control group, the changes of condylar process in 2 weeks occlusal trauma group were significantly prominent in anterior area: mastocyte regions were widen or disappearing, calcified cartilage regions were significantly widen, condylar cartilage cells were in disorder; the expression of DMP1-C increased significantly, and the expression of DMP1-N decreased significantly. Subchondral trabeculae was in no orderly pattern. The rats condylar cartilage mastocyte regions and the calcified cartilage regions in 2 weeks treatment group were gradually recovered, and the arrangement of condylar cartilage cells become regular and gradually distributed along the stress direction; the expression of DMP1-C was lower than 2 weeks occlusal traumatism group but higher than normal control group. There were no significant difference between 4 weeks treatment group and control group in the thickness of condylar cartilage, the layers of cells, the distribution of cartilage cell and the expression of DMP1-C and DMP1-N. Conclusion: Occlusal trauma accelerated the process of endochondral ossification in rats condylar process, with the changes of the expression of ECM mineralization protein DMP1-C and DMP1-N, simulating the treatment after occlusal adjustment, the tissue of condylar process, subchondral bone mineralization, and the expression of DMP1 could back to normal.

Key words: occlusal trauma, condyle, occlusal adjustment, DMP1, endochondral ossification

LIU Peihong, MA Su, LI Yongheng, WANG Haixia. Differential Expression of Extracellular Matrix Protein DMP1-C and DMP1-N in Condylar Cartilage Before and After Occlusal Trauma Treatment[J]. Journal of Oral Science Research, 2020, 36(5): 443-448.

References

[1] Clark GT, Tsukiyama Y, Baba K, et al. Sixty-eight years of experimental occlusal interference studies: what have we learned?[J]. J Prosthet Dent, 1999, 82(6): 704-713.
[2] Murphy MK, MacBarb RF, Wong ME, et al. Temporomandibular joint disorders: a review of etiology, clinical management, and tissue engineering strategies[J]. Int J Oral Maxillofac Implants, 2013, 28(6): e393-e414.
[3] Embree MC, Iwaoka GM, Kong D, et al. Soft tissue ossification and condylar cartilage degeneration following TMJ disc perforation in a rabbit pilot study[J]. Osteoarthritis Cartilage, 2015, 23(4): 629-639.
[4] Kong J, Yang Y, Sun S, et al. Effect of Toll-like receptor 4 on synovial injury of temporomandibular joint in rats caused by occlusal interference[J]. Mediators Inflamm, 2016, 2016: 7694921.
[5] Qin C, Brunn JC, Cook RG, et al. Evidence for the proteolytic processing of dentin matrix protein 1. Identification and characterization of processed fragments and cleavage sites[J]. J Biol Chem, 2003, 278(36): 34700-34708.
[6] Yang H, Zhang M, Wang X, et al. TNF accelerates death of mandibular condyle chondrocytes in rats with biomechanical stimulation-induced temporomandibular joint disease[J]. PLoS One, 2015, 10(11): e0141774.
[7] 魏佳琦,柳宏志,王天祥,等.咬合创伤对OVX小鼠牙周组织中HMGB1表达的影响[J]. 口腔医学研究, 2016, 32(6): 575-579.
[8] lzawa T, Mori H, Shinohara T, et al. Rebamipide attenuates mandibular condylar degeneration in a murine model of TMJ-OA by mediating a chondroprotective effect and by downregulating RANKL-mediated osteoclastogenesis[J]. PLoS One, 2016, 11(4): e0154107.
[9] Zhang J, Zhang HY, Zhang M, et al. Connexin43 hemichannels mediate small molecule exchange between chondrocytes and matrix in biomechanically-stimulated temporomandibular joint cartilage[J]. Osteoarthritis Cartilage, 2014, 22(6): 822-830.
[10] Zhang M,Wang H,Zhang J,et al. Unilateral anterior crossbite induces aberrant mineral deposition in degenerative temporomandibular cartilage in rats[J]. Osteoarthritis Cartilage, 2016, 24(5): 921-931.
[11] Sun Y, Chen L, Ma S, et al. Roles of DMP1 processing in osteogenesis, dentinogenesis and chondrogenesis[J]. Cells Tissues Organs, 2011, 194(2-4): 199-204.

[1]	WANG Dongxiang, WANG Guangsu, TANG Zhenglong, CHEN Youli, GAO Qiong, AN Ning. Expression of DKK-1/BMP-2 in the Healing of Mandibular High Condylar Fracture [J]. Journal of Oral Science Research, 2020, 36(8): 748-752.
[2]	LIU Geng, LI Le, XING Lu, FAN Xiangfei. Effect of β2-adrenergic Signal Transduction on Subchondral Bone Loss of Rat Mandibular Condyles Induced by Abnormal Molar Occlusion. [J]. Journal of Oral Science Research, 2020, 36(1): 28-32.
[3]	ZHENG De-Xin, LUO Dan, MAN Cheng, JIANG Hai-Tao, CHENG Jiao, GAO Si-Ji. Growth of Mandible after Condylar Reconstruction by Posterior Border of Mandibular Ramus [J]. Journal of Oral Science Research, 2019, 35(1): 60-63.
[4]	PI Na,HOU Min.. Research Progress on Effect of Different Lengthening Areas on the Temporomandibular Joint. [J]. Journal of Oral Science Research, 2018, 34(9): 936-938.
[5]	LEI Xin, DENG Shu-hai, GUAN Song-hua, HUANG Hui, CHEN Yu-ting, LIU Hong. Comparison of Spiral CT Three-Dimensional Reconstruction and MRI in Fracture of Mandibular Condyle [J]. Journal of Oral Science Research, 2018, 34(7): 746-750.
[6]	HUANG Shuo, LI Yong-feng, TAN Xin-ying, WANG Hong, ZHANG An-dong, HU Min, LIU Chang-kui. Study on Repair of Condyle Defect by Allograft Bone Combined with Marrow Mesenchymal Stem Cells. [J]. Journal of Oral Science Research, 2018, 34(5): 558-562.
[7]	LU Bin-hua, XI Wei-hong, LI Jian-fu, ZHOU Wu-chao, GAN Yan-zi. Design and Manufacture of An Improved Guiding Splint for Condylar Positioning. [J]. Journal of Oral Science Research, 2018, 34(3): 236-240.
[8]	LI Cheng, DENG Mo-hong, GUO Hui-lin, FENG Ya-ping, FANG Wei, LONG Xing. Influence of Hyaluronic Acid on HMGB-1 Expression of Chondrocytes with IL-1β Stimulation. [J]. Journal of Oral Science Research, 2018, 34(3): 241-244.
[9]	MIREGULI Tuersunjiang, LI Jian, LONG Xing, Ke Jin, MENG Qing-gong, CAI Heng-xing, WU Chun, XU Yu-li, RAN Ming, DENG Mo-hong. Application of Stabilization Splint on Remodeling of Condyle after Surgical Treatment with Temporomandibular Joint Disc Perforation. [J]. Journal of Oral Science Research, 2018, 34(3): 298-301.
[10]	LING Bin, WANG Bin, SHAO Bo, GONG Zhong-cheng. Diagnosis, Treatment, and Image Analysis of Condylar Osteochondroma. [J]. Journal of Oral Science Research, 2018, 34(3): 302-306.
[11]	TENG Bin-hong, ZHAO Yan-hong, YANG Qiang.. Advance in Tissue Engineering of Temporomandibular Joint Disc and Condyle. [J]. Journal of Oral Science Research, 2017, 33(6): 677-680.
[12]	LIU Qi, WEI Xiao-er, ZOU De-rong, YU Lv-feng.. Application of Mimics in Measurement of Condylar Volume and Surface Area. [J]. Journal of Oral Science Research, 2017, 33(4): 404-408.
[13]	WEI Jia-qi, LIU Hong-zhi, WANG Tian-xiang, LI Chao.. Effect of Occlusal Traumatism on HMGB1 in the Periodontal Tissues of Ovariectomized Mice: an Immunohistochemical Study. [J]. Journal of Oral Science Research, 2016, 32(6): 575-579.
[14]	XIAO Shen-zhao, REN Guang-hui, LIU Zhong-hao.. Recording Methods and Appraisal Systems of Centric Relation. [J]. Journal of Oral Science Research, 2016, 32(12): 1329-1332.
[15]	WANG Ya-fei, WANG Si-han, GUO Xiao-kai, et al. Effect of Different Frequency Tensile Stress on the Proliferation and Differentiation of Mandibular Condylar Chondrocytes [J]. Journal of Oral Science Research, 2015, 31(7): 662-665.