Study on Repair of Condyle Defect by Allograft Bone Combined with Marrow Mesenchymal Stem Cells.

doi:10.13701/j.cnki.kqyxyj.2018.05.023

Abstract

Abstract: Objective: To reconstruct condyle defect by allogeneic freeze-dried bone scaffold combined with bone marrow mesenchymal stem cells so as to provide experimental evidence for repairing of unilateral condyle defect. Methods: The left condyles from the condyle neck of 12 one year old beagles were cut off to prepare animal model of unilateral condyle defect. Then the animals were divided into two groups randomly: the experimental group with allergenic freeze-dried bone scaffold combined with bone marrow mesenchymal stem cells and the control group only with allergenic freeze-dried bone. The changes of opening degree were evaluated 4, 12, and 24 weeks after operation, and the morphological changes of condyle were observed. The animals were killed 24 weeks after the operation. The general observation, spiral CT scanning, micro-CT scanning, and histological examination were performed. Results: All allogeneic freeze-dried bone in the experimental group and control group could heal. After 24 weeks, the mouth opening degree of two groups of animals became almost normal. The condyle shape had no obvious change. The allograft bone in the experimental group was almost completely replaced by autogenous bone. In the control group, the new bone was formed mainly in the joint of the allograft bone and the autogenous bone. The bone mineral density and the quality of osteogenesis in the experimental group were better than those of the control group (P<0.05). Conclusion: Allogeneic bone scaffold combined with bone marrow mesenchymal stem cells can accelerate bone formation. Condylar shape changes little and the function recovery is good after 24 weeks. It lays a foundation for further study of allograft bone combined with bone marrow mesenchymal stem cells for clinical repair　of condyle defects.

Key words: Allogeneic freeze-dried bone, Bone marrow mesenchymal stem cells, Condyle defect, Tissue engineering, Reconstruction

HUANG Shuo, LI Yong-feng, TAN Xin-ying, WANG Hong, ZHANG An-dong, HU Min, LIU Chang-kui. Study on Repair of Condyle Defect by Allograft Bone Combined with Marrow Mesenchymal Stem Cells.[J]. Journal of Oral Science Research, 2018, 34(5): 558-562.

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