Effect of Calcium Silicate Incorporation on Collagen-chitosan Scaffold

doi:10.13701/j.cnki.kqyxyj.2019.07.023

Abstract

Abstract: Objective: To explore the effect of calcium silicate incorporation on collagen-chitosan scaffold. Methods: The scaffolds with 0%, 1%, 2%, 5%, and 8% (w/v) calcium silicate were prepared by freeze-drying method. The physicochemical properties of the scaffolds were tested by scanning electron microscopy, energy dispersive spectroscopy, and universal testing machine. The changes of biological properties were detected by simulating mineralization and cell culture in vitro. Results: With the increase of calcium silicate, the compressive elastic modulus and degradation rate of the scaffolds increased while the porosity decreased gradually compared to the control group (0% calcium silicate, P<0.05). The experimental group could promote the proliferation of osteoblasts and the expression of ALP (P<0.05). Moreover, varying amount of HA crystals were deposited in different experimental groups, while no HA crystal was deposited in the control group. Conclusion: Appropriate amount of calcium silicate can enhance the physicochemical properties of collagen-chitosan scaffold and promote osteogenesis and mineralization in vitro suggesting a potential scaffold for bone tissue engineering.

Key words: calcium silicate, collagen, chitosan , tissue engineering

LI Baoyin, BAN Shuofeng, DAI Huanyan, DUAN Congcong, XU Hongwei, HAN Bing. Effect of Calcium Silicate Incorporation on Collagen-chitosan Scaffold[J]. Journal of Oral Science Research, 2019, 35(7): 716-720.

References

[1] Grellier M, Bordenave L, Amédée J . Cell-to-cell communication between osteogenic and endothelial lineages:implications for tissue engineering [J]. Trends Biotechnol, 2009, 27(10):562-571.
[2] Herbert SP, Stainier DY. Molecular control of endothelial cell behaviour during blood vessel morphogenesis [J]. Nat Rev Mol Cell Biol, 2011, 12(9):551-564.
[3] Pei Y, Xin H, Shen J, et al. Development of a new pre-vascularized tissue-engineered construct using pre-differentiated rADSCs, arteriovenous vascular bundle and porous nano-hydroxyapatide-polyamide 66 scaffold [J]. BMC Musculoskelet Disord, 2013, 14:318
[4] Zhai W, Lu H, Chen L, et al. Silicate bioceramics induce angiogenesis during bone regeneration [J]. Acta Biomater, 2012, 8(1):341-349.
[5] Fielding GA, Bandyopadhyay A, Bose S. Effects of silica and zinc oxide doping on mechanical and biological properties of 3D printed tricalcium phosphate tissue engineering scaffolds [J]. Dent Mater, 2012, 28(2):113-122.
[6] 钟志华,施斌,周先略.磷酸三钙/壳聚糖支架与牙周膜干细胞混合培养的动物实验[J].口腔医学研究,2015,31(1):31-33.
[7] Arakawa C, Ng R, Tan S, et al. Photopolymerizable chitosan-collagen hydrogels for bone tissue engineering [J]. J Tissue Eng Regen Med, 2017, 11(1):164-174.
[8] Chaleawlert-Umpon S, Nuchuchua O, Saesoo S, et al. Effect of citrate spacer on mucoadhesive properties of a novel water-soluble cationic β-cyclodextrin-conjugated chitosan [J]. Carbohyd Polym, 2011, 84(1):186-194.
[9] 石珊珊,姚江武.壳聚糖-胶原复合TGF-β微球的复合支架的制备及用于牙周组织再生的可行性研究[J].口腔医学研究,2014,30(12):1138-1140.
[10] Kokubo T, Takadama H. How useful is SBF in predicting in vivo bone bioactivity [J]. Biomaterials, 2006, 27(15):2907-2915.
[11] Raschip IE, Vasile C, Macocinschi D. Compatibility and biocompatibility study of new HPC/PU blends [J]. Polym Int, 2010, 58(1):4-16.
[12] Dutta SR, Passi D, Singh P, et al. Ceramic and non-ceramic hydroxyapatite as a bone graft material: a brief review [J]. Ir J Med Sci, 2015, 184(1):101-106.
[13] Bhumiratana S, Grayson WL, Castaneda A, et al. Nucleation and growth of mineralized bone matrix on silk-hydroxyapatite composite scaffolds [J]. Biomaterials, 2011, 32(11):2812-2820.
[14] Li H, Chang J. Stimulation of proangiogenesis by calcium silicate bioactive ceramic [J]. Acta Biomater, 2013, 9(2):5379-5389

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