Effect of Slightly Acidic Electrolyzed Water on Oral Flora in Patients with Periodontitis by 16S rDNA Sequencing Analysis

doi:10.13701/j.cnki.kqyxyj.2020.11.007

Abstract

Abstract: Objective: To investigate the effect of slightly acidic electrolyzed water (SAEW) gargle on oral flora in patients with periodontitis. Methods: The saliva samples gargled with slightly acidic electrolyzed water of 10 patients with periodontitis who entered the supportive periodontal therapy were collected before and after 7 days. The total DNA of oral flora was extracted and sequenced by 16S rDNA sequencing, and the changes of oral flora diversity and structure were analyzed. Results: After gargled with slightly acidic electrolyzed water for 7 days, at phylum level, the relative abundance of proteobacteria, bacteroidetes and fusobacteria species was decreased. At the genus level, the relative abundance of periodontal pathogen genera-neisseria, porphyromonas, clostridium, and pretenella was decreased. Analysis of α-diversity: the Simpson index and Shannon index increased (P<0.05), Chao1 index, ACE index, and PD_whole_tree index were all increased (P<0.01). The analysis of the β-diversity index showed significant differences between the groups (P<0.01). Conclusion: The use of slightly acidic electrolyzed water can change the structure of oral flora and increase the abundance and diversity of oral flora in patients with periodontitis, which is conducive to the restoration of oral flora to the normal range.

Key words: slightly acidic electrolyzed water, 16S rDNA, oral flora, periodontitis

CAO Cheng, LI Ping, HU Zhenqing, LI Ziliang. Effect of Slightly Acidic Electrolyzed Water on Oral Flora in Patients with Periodontitis by 16S rDNA Sequencing Analysis[J]. Journal of Oral Science Research, 2020, 36(11): 1012-1015.

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