In Vitro Study of Modified Flowable Resin for Orthodontics

doi:10.13701/j.cnki.kqyxyj.2021.08.009

Abstract

Abstract: Objective: To evaluate the antibacterial activity and bond strength of flowing resin containing dimethylaminohexadecy methacrylate (DMAHDM). Methods: A modified orthodontic adhesive was prepared by adding 0% (control), 3%, 5%, and 7.5% (w/w) DMAHDM to 3M Z350XT flowable resin, recorded as group A, group B, group C, and group D. Enamel shear bond strength (SBS) was tested. The MTT method was used to detect the activity of the Streptococcus mutans biofilm. And bacterial adhesion was observed by scanning electron microscope. Data were analyzed with ANOVA and LSD-t statistical significance was set at the 0.05 probability level. Results: Group A showed no significant differences in the SBS compared with group B (P=0.600). However, the SBS values of group C and group D significantly decreased (P<0.05), although they could meet the orthodontic bonding requirements. The difference between DMAHDM weight concentration gradients was statistically significant (P=0.000), and group D had the best antibacterial effect. Conclusion: Mixing 7.5% DMAHDM into flowable resin improved the antibacterial properties and meet the orthodontic adhesive requirement.

Key words: orthodontic adhesive, antibacterial activity, white spot lesions, shear bond strength

QUAN Lulu, LI Xuewei, ZHAO Junjie, DENG Wenzhen, ZHANG Xu, DONG Wei, LIANG Yongqiang. In Vitro Study of Modified Flowable Resin for Orthodontics[J]. Journal of Oral Science Research, 2021, 37(8): 712-716.

References

[1] Bevinagidad S, Setty S, Patil A, et al. Estimation and correlation of salivary calcium, phosphorous, alkaline phosphatase, pH, white spot lesions, and oral hygiene status among orthodontic patients [J]. J Indian Soc Periodontol, 2020, 24(2):117-121.
[2] 买尔旦江·肉孜,胡首杉,曾福磊,等.正畸中釉质脱矿的控制与治疗研究进展[J].口腔医学,2020,40(9): 855-859.
[3] 张宁,张珂,白玉兴.抗菌性正畸粘接剂的研究进展[J].首都医科大学学报,2016,37(3):294-298.
[4] 张珂,张宁,徐华焜,等.含有新型季铵盐单体DMAHDM的抗菌正畸粘结剂[C]. 2016中国国际正畸大会暨第十五次全国口腔正畸学术会议.
[5] 李汉玲,陈然然.光固化流体树脂正畸托槽粘接剂对牙齿外观及抗剪切强度的影响分析[J].中国美容医学,2020,29(9):152-154.
[6] Antonucci JM , Zeiger DN , Tang K , et al. Synthesis and characterization of dimethacrylates containing quaternary ammonium functionalities for dental applications [J]. Dent Mater, 2012, 28(2):219-228.
[7] Cheng L, Weir MD, Zhang K, et al. Dental primer and adhesive containing a new antibacterial quaternary ammonium monomer dimethylaminododecyl methacrylate [J]. J Dent, 2013, 41(4):345-355.
[8] Cheng HY, Chen CH, Li CL, et al. Bond strength of orthodontic light-cured resin-modified glass ionomer cement [J]. Eur J Orthod, 2011, 33(2):180-184.
[9] Goracci C, Margvelashvili M, Giovannetti A, et al. Shear bond strength of orthodontic brackets bonded with a new self-adhering flowable resin composite [J]. Clin Oral Investig, 2013, 17(2):609-617.
[10] Tuncer C, Tuncer BB, Ulusoy C. Effect of fluoride-releasing light-cured resin on shear bond strength of orthodontic brackets [J]. Am J Orthod Dentofacial Orthop, 2009, 135(1):14.e1-e6.
[11] Zhang N, Ma J, Melo MA, et al. Protein-repellent and antibacterial dental composite to inhibit biofilms and caries [J]. J Dent, 2015, 43(2):225-234.
[12] Zhang N, Weir MD, Romberg E, et al. Development of novel dental adhesive with double benefits of protein-repellent and antibacterial capabilities [J]. Dent Mater, 2015, 31(7):845-854.
[13] Wang SP, Ge Y, Zhou XD, et al. Effect of anti-biofilm glass-ionomer cement on Streptococcus mutans biofilms.[J]. Int J Oral Sci, 2016, 8(2):76-83.
[14] Artun J, Brobakken BO. Prevalence of carious white spots after orthodontic treatment with multibonded appliances [J]. Eur J Orthod, 1986, 8(4):229-234.
[15] 李振霞,薛晶,陈婷婷,等.纳米二氧化钛对正畸粘接剂抗菌性与拉伸粘接强度的影响[J].口腔医学,2016,36(7):591-594.
[16] Lambert PA, Hammond SM. Potassium fluxes, first indications of membrane damage in micro-organisms [J]. Biochem Biophys Res Commun, 1973, 54(2):796-799.
[17] Luo S, Liu F, Yu B, et al. Preparation of low shrinkage stress Bis-GMA free dental resin composites with a synthesized urethane dimethacrylate monomer [J]. J Biomater Sci Polym Ed, 2019, 30(2):137-149.
[18] 黄颖虹,郑成,毛桃嫣,等.季铵盐型抗菌树脂合成及其漆膜的抗菌性能[J].化工学报,2015,66(S2):473-479.
[19] Wang X, Zhang N, Wang B, et al. Novel self-etching and antibacterial orthodontic adhesive containing dimethylaminohexadecyl methacrylate to inhibit enamel demineralization [J]. Dent Mater J, 2018, 37(4):555-561.
[20] Feng X , Zhang N , Xu HHK , et al. Novel orthodontic cement containing dimethylaminohexadecyl methacrylate with strong antibacterial capability [J]. Dent Mater J, 2017, 36(5):669-676.
[21] 张宁,马雁崧,徐华焜,等.季铵盐单体对正畸粘接剂粘接强度和抗菌性能的影响[J].北京口腔医学,2017,25(6):7-10.
[22] 仇元新,方绍伟,秦宗长.变异链球菌葡萄糖基转移酶转录调控因子的研究进展[J].口腔医学研究,2017,33(7):792-794.