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Spatio-temporal Variational Rules of Electric Signal in Palate Development of C57BL/6J Mouse
DUAN Shijun, DONG Shuxia, ZHENG Qian, WU Jun
2021, 37(8):
755-760.
DOI: 10.13701/j.cnki.kqyxyj.2021.08.018
Objective: To explore the spatio-temporal variational rules of electric signal in palate development of C57BL/6J mice, and assess the membrane potential changes of palate after selective inhibited KATP channel. Methods: DiBAC4(3) was used to trace the electric signal of embryonic palate through conversion from biological signal to optical signal with histological examination. Then the embryonic palate was cultured in vitro and treated with a selective K(ATP) channel inhibitor (HMR1098) for 24, 48, and 72 hours. The expression levels of TGF-β3 during palatogenesis were examined by immunohistochemistry analysis. Results: The oral, nasal, and medial edge epithelium existed obvious electrical signal during the four palatal development phases. The electrical signal intensity of medial edge epithelium decreased gradually along with palatogenesis. After treated by HMR1098, the palatal process could still elevate, contact, and fuse in vitro, however, the form of palatal process was slenderer and sharper. The TGF-β3 was strongly expressed in the oral, nasal, and medial edge epithelium after treated for 24h. With the increase of the HMR1098 treatment time, the expression of TGF-β3 trended to decline, eventually disappear. Conclusion: There were spatio-temporal variation of electric signal during palatogenesis, and the endogenous electrical fields affected the development of palate.
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