Effects of Periodontitis on Plasma Trimethylamine N-Oxide in Mice Fed with High-fat Diet

doi:10.13701/j.cnki.kqyxyj.2023.01.014

Abstract

Abstract: Objective: To evaluate the effects of periodontitis on plasma trimethylamine N-oxide (TMAO) production in mice fed a high-fat diet and to explore the role of gut microbiota. Methods: Ten wild-type male C57BL/6J mice were randomly divided into 2 groups: (1) high-fat diet group (HFD) and (2) high-fat diet + periodontitis group (HFD_PD). Eight weeks later, the mice were sacrificed to detect the alveolar bone loss, the concentration of plasma TMAO, the relative gene expression in liver and gut, and microbial communities in cecal contents. Results: Compared with HFD group, the concentration of plasma TMAO was significantly increased in HFD_PD group (P<0.01). At the phylum level, the abundance of Firmicutes and Desulfobacterota decreased and the abundance of Bacteroidota and Campilobacterota increased (P<0.05). Pearson correlation analysis showed that Eubacterium_coprostanoligenes and Lachnospiraceae_NK4A136 in HFD_PD group were positively correlated with plasma TMAO levels, while Muribaculaceae was negatively correlated with plasma TMAO levels (P<0.05). The expression of IL-6 in intestinal tissue was increased (P<0.01), while the expression of ZO-1 was decreased (P<0.01). The expression levels of IL-6 and TNF-α in liver were significantly increased (P<0.01, P<0.001). Conclusion: Periodontitis can increase the concentration of plasma TMAO in high-fat diet mice, which may be achieved by regulating gut microbiota related to TMAO metabolism.

Key words: periodontitis, trimethylamine N-oxide, gut microbiota

XIE Weige, DONG Jingwen, HUANG Lingyan, KANG Wen, WANG Lu, WU Juan, XIE Sijing. Effects of Periodontitis on Plasma Trimethylamine N-Oxide in Mice Fed with High-fat Diet[J]. Journal of Oral Science Research, 2023, 39(1): 74-80.

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