Effects of Porphyromonas Gingivalis GroEL on Human Umbilical Vein Endothelial Cells.

doi:10.13701/j.cnki.kqyxyj.2022.09.016

Abstract

Abstract: Objective: To investigate the effects of GroEL, a metabolite of Porphyromonas gingivalis(P.g) , on the proliferation viability, apoptosis, and inflammation response of human umbilical vein endothelial cell (HUVEC) in vitro. Methods: The HUVEC were stimulated with different concentrations of P.g GroEL (2, 4, 6, 8, 10 μg/mL) for 24 h to observe the morphologic changes. The proliferation viability of HUVEC was detected by CCK-8. Apoptosis rate of HUVEC was detected by flow cytometry. The expressions of interleukin-6 (IL-6), interleukin-8 (IL-8), intracellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin were detected by RT-qPCR and ELISA, respectively. Results: P.g GroEL significantly decreased HUVEC viability, and apoptosis rate was increased at 24 h (P<0.05). The inhibitory effect of P.g GroEL and apoptosis rate was more obvious as the concentration increased. P.g GroEL also promoted the expression of IL-6, IL-8, ICAM-1, VCAM-1, and E-selectin (P<0.05). Conclusion: P.g GroEL may play an important role in the development of atherosclerosis promoted by periodontitis.

Key words: Porphyromonas gingivalis, GroEL, periodontitis, human umbilical vein endothelial cell, atherosclerosis

YAN Rui, GUO Kaili, CAO Fengdi, ZHANG Jun, MA Zhe. Effects of Porphyromonas Gingivalis GroEL on Human Umbilical Vein Endothelial Cells.[J]. Journal of Oral Science Research, 2022, 38(9): 872-876.

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