Role and Mechanism of Porphyromonas Gingivalis in the Differentiation of Osteoclast Precursor

doi:10.13701/j.cnki.kqyxyj.2022.01.014

Abstract

Abstract: Objective: To investigate the role and mechanism of Porphyromonas gingivalis(P.g)in the differentiation of osteoclast precursors (OCPs) through in vitro direct co-culture or in vivo remote local stimulation. Methods: The OCPs were isolated from 6 to 8-week-old male C57BL/6 mice femurs and cultured in vitro. The OCPs were direct co-cultured with different multiplicity of infection P.g for 6 hours. Then the viability and the ratio of subtypes of OCPs in each group were detected and compared. After removing P.g, the OCPs continued to be cultured to induce osteoclastogenesis. Bone defects were created on the right femurs of the mice, and the suspension with or without P.g was injected into the defects twice a week for 4 weeks. The OCPs from left femurs of mice were isolated, cultured, and induced osteoclastogenesis. After culturing OCPs for 4 or 6 days, the number of mature osteoclasts and the expression levels of related genes and proteins in each group were detected and compared. Results: Direct in vitro co-culture of P.g and OCPs did not affect the activity and the proportion of subtypes of OCPs, but inhibited the osteoclastogenesis of OCPs which was related to the declining expression of osteoclast-related genes and proteins. The remote in vivo local stimulation of P.g up-regulated the expression levels of some osteoclast-related and inflammation-related genes, but did not affect the osteoclastogenesis of OCPs. Conclusion: Direct in vitro co-culture of P.g and OCPs can inhibit the osteoclastogenesis of OCPs, while in vivo local stimulation does not affect the osteoclastogenesis of OCPs in other parts of the body.

Key words: Porphyromonas gingivalis, osteoclast precursors, osteoclast, direct co-culture, in vivo local stimulation

WU Shuxuan, TAN Jingjing, LI Feng, WAN Qilong. Role and Mechanism of Porphyromonas Gingivalis in the Differentiation of Osteoclast Precursor[J]. Journal of Oral Science Research, 2022, 38(1): 65-70.

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