A Study on Activation of Autophagy in Human Periodontal Ligament Cells by Reactive Oxygen Species in Starvation Environment.

doi:10.13701/j.cnki.kqyxyj.2022.09.017

Abstract

Abstract: Objective: To study the changes of autophagy levels of human periodontal ligament cells (hPDLCs) under starvation conditions, and to explore the role of reactive oxygen species (ROS) in the process of autophagy. Methods: hPDLCs were isolated and cultured. EBSS was applied to simulate a nutrient-deficient environment. Transmission electron microscopy was used to observe the production of autophagosomes to evaluate the autophagy level in hPDLCs. In order to explore the potential regulatory mechanism of autophagy activation under starvation conditions, hPDLCs were pretreated with antioxidants N-acetylcysteine (NAC) to inhibit the generation of ROS. DCFH-DA staining was used to detect the level of ROS, and the intensity of autophagy were monitored by RT-qPCR and Western blot which measure the expression of autophagy-related proteins LC3-Ⅱ, Beclin-1, and p62. Results: After EBSS starvation, the autophagy level increased. In addition, NAC partially reversed the autophagy levels in starved hPDLCs by inhibiting ROS production. Conclusion: ROS act as a signal molecule to mediate autophagy. Starvation activates autophagy in hPDLCs by inducing ROS generation which protects cells from nutritional deficiency.

Key words: reactive oxygen species, autophagy, starvation, N-acetylcysteine

HE Haiyan, YU Jinghong, WU Yujie, Zhang Min, XU Xiaomei. A Study on Activation of Autophagy in Human Periodontal Ligament Cells by Reactive Oxygen Species in Starvation Environment.[J]. Journal of Oral Science Research, 2022, 38(9): 877-881.

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