Inhibition of Human Dental Pulp Viability by 2-Hydroxyethyl Methacrylate.

doi:10.13701/j.cnki.kqyxyj.2018.04.012

Abstract

Abstract: Objective: To investigate the toxicity and possible mechanism of 2-hydroxyethyl methacrylate on human dental pulp in tooth slice organ culture model. Methods: Human tooth slice organ culture model was established in vitro, which was exposed to 8mmol/L HEMA for 12 h, 24 h, and 48 h. Separated dental pulp tissue viability was detected by CCK-8 assessment. Hematoxylin and eosin (H&E) staining demonstrated the cell density change of dental pulp tissue from the ex vivo cultured tooth slice organ model. TEM analysis showed the ultrastructure change of dental mesenchymal cells during autophagy process. Immunostaining detected the LC3 expression in dental pulp. Result: 8 mmol/L HEMA exposure decreased the viability and cell density of dental pulp tissue and increased autophagic vacuoles in a time-dependent manner. And the expression of autophagic biomarkers LC3 increased after 48 h. Conclusion: HEMA greatly decreased the tissue viability and induced autophagy in human dental pulp.

Key words: 2-hydroxyethyl methacrylate, Tooth slice organ, Dental pulp toxicity, Autophagy

ZHU Shen-ting, YU Jing-jing, PENG Bin.. Inhibition of Human Dental Pulp Viability by 2-Hydroxyethyl Methacrylate.[J]. Journal of Oral Science Research, 2018, 34(4): 384-387.

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