Effect of Liquid Phase Concentrated Growth Factor Combined with Hyaluronic Acid Injection on Temporomandibular Joint Osteoarthritis

doi:10.13701/j.cnki.kqyxyj.2023.04.016

Abstract

Abstract: Objective: To investigate the effect of autologous liquid phase concentrated growth factor (LPCGF) combined with hyaluronic acid (HA) injection in the treatment of rat temporomandibular joint osteoarthritis (TMJOA). Methods: A total of 33 SD rats were used in the present study, including blank control group (n=3) and experimental group (n=30). Type Ⅱ collagenase was injected into bilateral TMJ cavity to establish TMJOA model. Three rats were randomly selected as the untreated group from experimental group, while the rest of experimental group was selected as treated group and was randomly divided into three subgroups, including HA group (A), LPCGF group (B), and combined treatment group (C). Each group was treated with intra-articular injection once a week, and the tissue samples of TMJ were acquired from three rats randomly every week for 2, 4, and 6 weeks after treatment. HE staining and Safranin O-fast green staining were performed to observe histological changes of the joints and evaluate according to the modified Mankin's score. Immunohistochemistry was performed to detect the changes of interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α) in condylar cartilage. Results: Intra-articular injection of type II collagenase could successfully induce the rat TMJOA model. Histological observation revealed the repair of condylar cartilage and subchondral bone after injection treatment. The modified Mankin’s score was reduced in all treated groups (P>0.05). The immunohistochemical results showed that the expression of IL-1β and TNF-α in TMJ cartilage could be significantly inhibited by HA or LPCGF injection treatment, and the combined treatment group had the most significant inhibition effect (P<0.05). Conclusion: Both LPCGF and HA can treat temporomandibular joint osteoarthritis in rats. However, LPCGF combined with HA injection has the most significant effect on the modification of condylar and inhibition of IL-1 and TNF-α in articular cartilage (P<0.05).

Key words: temporomandibular joint osteoarthritis, hyaluronic acid, concentrated growth factor, interleukin-1β, tumor necrosis factor-α

ZHAO Chen, JIANG Shanshan, LIU Yang, WU Suran, QIU Tong, BAI Yuehui, WU Jie, JIA Yujing. Effect of Liquid Phase Concentrated Growth Factor Combined with Hyaluronic Acid Injection on Temporomandibular Joint Osteoarthritis[J]. Journal of Oral Science Research, 2023, 39(4): 362-369.

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