Effect of Occlusal Interference on Endoplasmic Reticulum Stress and ER Stress-mediated Apoptosis in Condylar Chondrocytes of Rat.

Abstract

Abstract: Objective: To study the effects of occlusal interference on endoplasmic reticulum stress and ER stress-mediated apoptosis in mandibular condylar chondrocytes of Sprague-Dawley (SD) rat. Methods: In experimental groups,the malocclusion was created by bonding the harmful prosthesis on first molar of upper jaw. Animals were sacrificed at 4, 8 and 12 weeks later. Toluidine blue and hematoxylin-eosin (HE) staining were carried out to study the morphological changes of the condylar cartilage.The ultrastructure of chondrocyte was observed by transmission electron microscopy. Immunohistochemical(IHC)staining was performed to detect the levels of expression of GRP78 and Caspase-12 in the condylar cartilages. Results: In the condylar cartilages of experimental groups, the matrix was degraded and the chondrocytes density decreased significantly. Endoplasmic reticulum expanded and fractured into parts. Comparing to the control group, the expression of GRP78 increased in all time points (P<0.05,P<0.05,P<0.01), while the expression of Caspase-12 increased at the end of 8 and 12-weeks. Conclusion: The endoplasmic reticulum stress and ER stress-mediated apoptosis occurred in the condylar chondrocyte of rat with occlusal interference, which should play an important role in the degradation of condylar cartilage.

Key words: Occlusion , Temporomandibular joint (TMJ), Osteoarthritis (OA), Endoplasmic reticulum stress, ER stress-mediated apoptosis

CLC Number:

R782.6

CAI Chang-an, ZHANG Li. Effect of Occlusal Interference on Endoplasmic Reticulum Stress and ER Stress-mediated Apoptosis in Condylar Chondrocytes of Rat.[J]. Journal of Oral Science Research, 2015, 31(1): 23-26.

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