Study on the Mutual Induction between Tooth Germ Cells and Bone Marrow Mesenchymal Stem Cells Transfected by Bone Morphogenetic Protein4 Gene in Vitro.

Abstract

Abstract: Objective: To study the mutual induction between tooth germ cells and bone marrow mesenchymal stem cells with bone morphogenetic protein4 gene in vitro. Methods: Lentiviral vectors carrying BMP4 were constructed to transfect rat bone marrow mesenchymal stem cells(BMSCs) and BMSCs tansfected by BMP4 were co-cultured with tooth germ cells (1:1)respectively .The cells were divided into five groups: BMSCs group, BMP4/BMSCs group, tooth germ cells group, BMSCs tooth germ cells group (the mixed group1), BMP4/BMSCs tooth germ cells group (the mixed group2) .The five groups were extracted using real-time quantitative PCR and western blotting to detect the mRNA and protein levels of collagen I, enamel protein,dentin matrix protein 1, and homeobox gene 1. Results: In the mixed group2, mRNA and protein levels of collagen I, enamel protein,dentin matrix protein 1 and homeobox gene 1 were higher, and the difference was statistically significant (P<0.05). Conclusion: The co-culture of tooth germ cells and BMSCs tansfected by BMP4 can increase the expressions of odontogenic genes,and they are suitable as seed cells of tissue-engineered tooth.

Key words: Tooth germ cells, Bone mesenchymal stem cells, Bone morphogenetic protein4, Real-time fluorescent quantitative , PCR , Western-blotting

CLC Number:

Q132

WANG Huan-huan, SHEN Yu-feng, HU Yang, et al.. Study on the Mutual Induction between Tooth Germ Cells and Bone Marrow Mesenchymal Stem Cells Transfected by Bone Morphogenetic Protein4 Gene in Vitro.[J]. Journal of Oral Science Research, 2015, 31(3): 237-241.

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