Identifying Molecular Markers in Oral Squamous Cell Carcinoma Originating from Oral Submucous Fibrosis using QPCR

doi:10.13701/j.cnki.kqyxyj.2017.10.002

Abstract

Abstract: Objective: To explore the specific molecular markers of oral squamous cell carcinoma (OSCC) originating from oral submucous fibrosis (OSF), and provide theoretical foundation for clarifying the key mechanism and early diagnosis of OSCC. Methods: Based on the analysis data of early gene chip, 10 candidate genes CXCL8, CXCL1, PTPRZ1, WDR66, DKK1, COX6A2, ATP1A2, TPM1, ABCA8 and MIB1 were screened out. Real-time reverse transcription-polymerase chain reaction (QPCR) was used to detect the expression of candidate genes in 12 OSCC samples originating from OSF, 10 non-OSF OSCC samples, and 8 samples from normal tissues. The results were compared and analyzed using 2^-ΔΔCTequation.Results: The expression of CXCL1 and PTPRZ1 were significantly higher than that of the non-OSF and normal control group (P<0.05). The expression of TPM1 was significant lower than that of the non-OSF and normal control group (P<0.05). Conclusion: CXCL1, PTPRZ1, and TPM1 are meaningful to the early diagnosis of OSCC originating from OSF, expected to become specific molecular markers of the early diagnosis.

Key words: Oral submucous fibrosis , Oral squamous cell carcinoma, QPCR

CLC Number:

R739.8

WANG Yuan ,JIAN Xin-chun ,LIU De-yu. Identifying Molecular Markers in Oral Squamous Cell Carcinoma Originating from Oral Submucous Fibrosis using QPCR[J]. Journal of Oral Science Research, 2017, 33(10): 1027-1030.

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