Abstract: Objective: To evaluate the effects of layer-by-layer self-assembly technique and micro-arc oxidation surface on bone integration of pure titanium implant. Methods: Twenty-four New Zealand white rabbits were selected to construct animal models. Two, 4 and 6 weeks after operation, CBCT and implant bone torsion output test were performed to observe osseointegration of implants. Six weeks after operation, scanning electron microscopy (SEM) was used to observe the surface morphology of implants. Results: CBCT results showed that at post-operative 2, 4 and 6 weeks, the healing of bone defect was good in the sequence of group C, group B and group A. At post-operative 6 weeks, there were no obvious dash areas on implant bone interface, and the density was similar to normal bone. Implant bone torsion output test results showed that there were no obvious differences at post-operative 2 weeks in torsion. At post-operative 4 weeks, the torsion was higher in group B and C compared to group A. At post-operative 6 weeks, the torsion was the highest in group C compared to group A and B. Moreover, at post-operative 6 weeks, osseointegration was significantly increased in group C. Conclusion: TA2-MAO-NAOH-CS coating and TA2-MAO-NAOH-CS-ALG coating could promote the bone integration, and the effects of TA2-MAO-NAOH-CS-ALG coating were better.

Key words: Titanium implant , Layer-by-layer self-assembly technique, CS, ALG, Animal model