Effects of Ursolic Acid on Proliferation and Mineralization of Cementoblasts

doi:10.13701/j.cnki.kqyxyj.2016.12.002

Abstract

Abstract: Objective: To investigate the effect of ursolic acid on murine cementoblasts cell line OCCM-30 in vitro. Methods: Cementoblasts were incubated with ursolic acid at various concentrations. Proliferation was detected using cck-8 assay. Cell cycle and apoptosis were checked by flow cytometry analysis. The activity of alkaline phosphatase was tested by enzyme-linked method on microplate. The mineralization was tested by alizarin red S staining. Results: Compared with the control group, ursolic acid (2.5μmol/L) increased the cell proliferation rate and proliferation index of the cell cycle, reduced the early apoptotic rate, up-regulated the activity of alkaline phosphatase and the quantity of the mineralized nodules. Conclusion: Ursolic acid can promote the proliferation of cementoblast, enhance the activity of alkaline phosphatase, and facilitate the differentiation.

Key words: Ursolic acid , Murine cementoblasts cell line OCCM-30, Root absorption and repair

CLC Number:

R783.5

WANG Yu-zhuo, BAO Xing-fu, JIANG Huan, GAO Shang, LIU Jin-feng, WANG Chun-hui, HU Min. Effects of Ursolic Acid on Proliferation and Mineralization of Cementoblasts[J]. Journal of Oral Science Research, 2016, 32(12): 1229-1233.

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