口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (10): 1051-1055.DOI: 10.13701/j.cnki.kqyxyj.2016.10.012

• 临床研究论著 • 上一篇    下一篇

口腔黏膜癌变过程中Bmi-1及P16蛋白的表达及意义

李咏1, 王春风1, 赵光叶1, 刘一龙1, 黄元清1*, 蔡杨2*   

  1. 1. 湖南医药学院口腔医学系 湖南 怀化 418000;
    2. 贵州医科大学附属医院口腔内科 贵州 贵阳 550004
  • 收稿日期:2016-03-23 出版日期:2016-10-26 发布日期:2016-10-26
  • 通讯作者: 黄元清,电话:0745-2373365;蔡扬,电话:0851-6774432
  • 作者简介:李咏(1983~ ),女,湖北人,硕士,主治医师,主要从事口腔科临床治疗工作。
  • 基金资助:
    教育部留学回国人员科研流动基金(编号:N2005-1);国家自然科学基金(编号:30460139);湖南省教育厅科学研究项目(编号:14B141)

Expression and Significance of Bmi-1 and P16 in Oral Carcinogenesis.

LI Yong1, WANG Chun-feng1, ZHAO Guang-ye1, LIU Yi-long1, HUANG Yuan-qing1*, CAI Yang2*.   

  1. 1. Department of Stomatology, Hunan University of Medicine, Huaihua 418000, China;
    2. Department of Oral Medicine, the Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China.
  • Received:2016-03-23 Online:2016-10-26 Published:2016-10-26

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摘要： 目的:了解Bmi-1及P16蛋白在口腔黏膜癌变过程中的表达情况,探讨Bmi-1-P16通路在口腔鳞癌发生发展中的作用及意义。方法::正常口腔黏膜10例、上皮异常增生31例、口腔鳞癌61例的石蜡包埋组织,采用免疫组化SP法检测Bmi-1及P16表达情况,分析二者的相关性及其临床病理学意义。结果:Bmi-1在正常口腔黏膜、上皮异常增生及OSCC组织中的阳性率分别为0%、29%(9/31)和62.3%(38/61);P16在正常、上皮异常增生及OSCC组中的阳性率分别为100%(10/10)、77.4%(24/31)和47.5%(29/61)。OSCC中Bmi-1阳性率在P16阴性组高于P16阳性组,差异有统计学意义(P<0.05),Spearman相关分析显示两者间具负相关关系(r=-0.414,P<0.05)。Bmi-1阳性表达及Bmi-1高表达且P16失表达与OSCC临床分期及淋巴结转移密切相关(P<0.05)。结论:Bmi-1过表达是口腔黏膜癌变过程中的早期事件,口腔鳞癌中Bmi-1过表达可能与P16失表达有关并与临床分期及淋巴结转移密切相关,有可能作为临床评估口腔鳞癌浸润、转移和预后的参考指标之一。

关键词: B细胞特异性莫洛氏鼠白血病病毒插入位点-1, P16蛋白, 癌前损害, 口腔鳞状细胞癌

Abstract: Objective: To investigate the expression of Bmi-1 and P16 proteins in oral precancerous lesions and oral squamous cell carcinomas (OSCC), and elucidate their possible roles pathway in oral carcinogenesis.Methods: The tissues of 10 cases of normal oral epithelium, 31 cases of different-degree epithelium dysplasia and 61 cases of OSCC with different differentiation were collected and investigated for the expression of Bmi-1 and P16 proteins by using immunohistochemistry. The clinical and pathological significance of Bmi-1 over-expression in oral carcinogenesis was statistically analyzed by SPSS11.5.Results: The percentages of positive staining for Bmi-1 were 0 % in normal oral mucosa, 29% (9/31) in epithelial dysplasia and 62.3% (38/61) in OSCC, respectively. The percentages of positive staining for P16 were 100% (10/10) in normal oral mucosa, 77.4% (24/31) in epithelial dysplasia and 47.5% (29/61) in OSCC, respectively. The percentage of Bmi-1 over-expression with negative P16 staining in OSCC was significantly higher than that in OSCC with positive P16 staining (P<0.05), and there was a negative correlation between the expression of Bmi-1 and P16 in OSCC (r=-0.414, P<0.05). In addition, the over-expression of Bmi-1 and low-expression of P16 were positively correlated with TNM stage and lymph node metastasis in OSCC (P<0.05).Conclusion: Up-regulation of Bmi-1 was an early event in oral carcinogenesis, and might be caused by the down-regulation of P16 in OSCC, closely correlated with TNM stage and lymph node metastasis. Bmi-1 could be used as a marker for assessing the progression and prognosis of OSCC patients.

Key words: Bmi-1, P16, Precancerous lesion, Oral squamous cell carcinoma

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