口腔医学研究

口腔医学研究 ›› 2023, Vol. 39 ›› Issue (11): 1012-1017.DOI: 10.13701/j.cnki.kqyxyj.2023.11.014

• 口腔生物学研究 • 上一篇    下一篇

双相磷酸钙/聚醚醚酮成骨性能的研究

顾腾1, 罗桂生1, 徐先知1, 李俊俊1, 王旭冉1, 袁长永1,2, 王鹏来1,2*   

  1. 1.徐州医科大学口腔医学院 江苏 徐州 221004;
    2.徐州医科大学附属口腔医院种植科 江苏 徐州 221000
  • 收稿日期:2023-06-13 出版日期:2023-11-28 发布日期:2023-11-22
  • 通讯作者: *王鹏来,E-mail:wpl0771@163.com
  • 作者简介:顾腾(1998~ ),男,南京人,硕士,研究方向:种植体表面改性。
  • 基金资助:
    徐州市科技项目(编号:KC21151)江苏省研究生科研与实践创新计划项目(编号:KYCX22_2973)

Osteogenic Properties of Polyetheretherketone Biocomposited by Biphasic Calcium Phosphate

GU Teng1, LUO Guisheng1, XU Xianzhi1, LI Junjun1, WANG Xuran1, YUAN Changyong1,2, WANG Penglai1,2*   

  1. 1. School of Stomatology, Xuzhou Medical University, Xuzhou 221004, China;
    2. Department of Oral Implantology, Affiliated Stomatological Hospital of Xuzhou Medical University, Xuzhou 221000, China
  • Received:2023-06-13 Online:2023-11-28 Published:2023-11-22

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摘要： 目的: 研究经双相磷酸钙改性后的聚醚醚酮复合材料的成骨性能。方法: 经复合工艺加工的双相磷酸钙/聚醚醚酮复合材料作为实验组,聚醚醚酮作为对照组。利用扫描电子显微镜、X射线光电子能谱仪、接触角测量仪检测样品表面形状、化学成分组成和亲水性。将大鼠骨髓间充质干细胞与样品共培养,利用CCK-8实验检测细胞增殖特性,通过实时荧光定量PCR检测成骨基因OCN、ALP、BMP-2、RUNX2、SP7的表达。此外,通过建立大鼠股骨植入模型,将两组种植体植入大鼠股骨远端,采用Micro-CT分析种植体骨结合情况。结果: 经双相磷酸钙改性后的聚醚醚酮材料表面形貌虽然未发生明显变化,但成功引入了骨生长不可或缺的钙、磷元素,并提高了样品表面亲水性(P<0.01)。改性后的聚醚醚酮显著促进了细胞增殖活性(P<0.01),表现出了良好的生物安全性,也改善了细胞对成骨基因ALP、BMP-2、OCN、RUNX2、SP7的表达(P<0.05)。在大鼠体内实验中,双相磷酸钙/聚醚醚酮种植体表面拥有更多、致密的新骨形成(P<0.01)。结论: 经过双相磷酸钙改性后的聚醚醚酮明显改善了聚醚醚酮表面生物活性,提高了亲水性能,并可以促使成骨细胞的分化、增殖,从而提高种植体骨结合率。

关键词: 聚醚醚酮, 骨结合, 双相磷酸钙, 复合材料

Abstract: Objective: To investigate the osteogenic properties of polyetheretherketone modified by the biphasic calcium phosphate. Methods: The biphasic calcium phosphate/polyetheretherketone composites processed by the composite process were used as the experimental group and polyetheretherketone as the control group. Scanning electron microscopy, X-ray photoelectron spectroscopy, and contact angle measurement were used to examine the surface morphology, chemical elemental composition, and hydrophilicity of the samples. Rat bone marrow mesenchymal stem cells were co-cultured with the samples, and the proliferation performance of the cells was detected by CCK-8 assay; the expression of osteogenic genes OCN, ALP, BMP-2, RUNX2, and SP7 were detected by real-time fluorescence quantitative PCR. In addition, two groups of implants were implanted into the distal femur of rats by establishing a rat femoral implant model, and the osseointegration of the implants was analyzed by Micro-CT. Results: Although the surface morphology of the polyetheretherketone material modified by biphasic calcium phosphate did not change significantly, calcium and phosphorus, which were essential for bone growth, were successfully introduced and the surface hydrophilicity of the samples was improved (P<0.01). The modified polyetheretherketone significantly promoted cell proliferation activity (P<0.01) and showed good biosafety; and it also improved the expression of the osteogenic genes ALP, BMP-2, OCN, RUNX2, and SP7 (P<0.05). In vivo experiments in rats, the surface of the biphasic calcium phosphate/polyetheretherketone implants possessed more and denser new bone formation (P<0.01). Conclusion: Polyetheretherketone modified with biphasic calcium phosphate significantly improved the bioactivity of the polyetheretherketone surface, enhanced hydrophilic properties, and was able to promote osteoblast differentiation and proliferation and enhance the osseointegration properties of the implants.

Key words: polyetheretherketone, osseointegration, biphasic calcium phosphate, composite materials