口腔医学研究

口腔医学研究 ›› 2026, Vol. 42 ›› Issue (5): 378-385.DOI: 10.13701/j.cnki.kqyxyj.2026.05.004

• 口腔材料学研究 • 上一篇    下一篇

EGCG壳聚糖纳米粒改性GIC的物理特性及抗菌性能的评价

陈海霖1, 张雪琼1, 蔡佳婧1, 胡晨1, 吴茜2*   

  1. 1.武汉理工大学化学化工与生命科学系 湖北 武汉 430070;
    2.湖北省妇幼保健院口腔科 湖北 武汉 430070
  • 收稿日期:2025-11-25 出版日期:2026-05-28 发布日期:2026-05-25
  • 通讯作者: *吴茜,E-mail:larkymolar@163.com
  • 作者简介:陈海霖(2001~ ),女,成都人,硕士在读,主要从事药物新剂型研究。
  • 基金资助:
    湖北省自然科学基金(编号:2023AFC043)

Evaluation of Physical and Antibacterial Properties of Glass Ionomer Cement Modified by EGCG-loaded Chitosan Nanoparticles

CHEN Hailin1, ZHANG Xueqiong1, CAI Jiajing1, HU Chen1, WU Qian2*   

  1. 1. Department of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China;
    2. Department of Stomatology, Hubei Maternity and Child Health Hospital, Wuhan 430070, China
  • Received:2025-11-25 Online:2026-05-28 Published:2026-05-25

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摘要： 目的:探究表没食子儿茶素没食子酸酯壳聚糖纳米粒(epigallocatechingallate-chitosan nanoparticles, EGCG-NPs)掺入玻璃离子水门汀(glass ionomer cement, GIC)提升其物理性能和抗菌性能的最佳整合浓度。方法:采用离子交联法制备EGCG-NPs。以纳米粒形态、粒径分布、Zeta电位、多分散系数和释放研究来表征纳米粒;以材料的抗压强度、老化性能、吸水溶解性、氟离子释放、抗菌性能为指标,按照掺入不同质量分数(0.5、1、2、3、4、5 wt.%)的EGCG-NPs制备试样并进行筛选,并进行细胞毒性评价。结果:在28 d内,随着EGCG-NPs添加量的增加,抗压强度先增大后减小,在1 wt.%达到峰值;材料的吸水溶解率随EGCG-NPs添加量的增加影响增大;GIC抗菌性能和氟离子释放水平的强弱与添加EGCG-NPs的浓度呈正比;各组材料均无细胞毒性。结论:添加1 wt.%的EGCG-NPs能最大限度提高GIC的抗压强度和抗菌能力,显著降低GIC的溶解率,不影响吸水率,有提升氟离子释放的趋势。

关键词: 表没食子儿茶素没食子酸酯, 纳米粒子, 壳聚糖, 玻璃离子水门汀

Abstract: Objective: To explore the optimal integration concentration of epigallocatechin gallate-chitosan nanoparticles (EGCG-NPs) incorporated into glass ionomer cement (GIC) for enhancing its physical and antibacterial properties. Methods: EGCG-NPs were prepared via the ionic crosslinking. The nanoparticles were characterized by morphology, particle size distribution, zeta potential, polydispersity index (PDI), and release behavior. Specimens were prepared by adding EGCG-NPs at different mass fractions (0.5, 1, 2, 3, 4, 5 wt.%) and screened based on indicators including compressive strength, aging resistance, water absorption and solubility, fluoride ion release, and antibacterial activity. Cytotoxicity evaluation was also performed. Results: Within 28 days, the compressive strength of GIC first increased and then decreased with the increase of EGCG-NPs content, reaching a peak at 1 wt.%. The water absorption and solubility of the material were increasingly affected by the addition of EGCG-NPs. The antibacterial activity and fluoride ion release level of GIC were positively correlated with the concentration of EGCG-NPs. All groups of materials showed no cytotoxicity. Conclusion: The addition of 1 wt.% EGCG-NPs can maximize the compressive strength and antibacterial ability of GIC, significantly reduce its solubility (P<0.05), without affecting water absorption, and exhibit a trend of enhancing fluoride ion release.

Key words: epigallocatechin gallate, nanoparticles, chitosan, glass ionomer cement