茶多酚对内毒素作用下人牙周膜细胞分泌和表达Toll样受体4的影响

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (2): 140-143.

茶多酚对内毒素作用下人牙周膜细胞分泌和表达Toll样受体4的影响

孔宁静^1,2,李小娜¹,范芹^1,2,管晓燕^1,2,白国辉^1,2,刘建国^1,2*

1. 遵义医学院口腔学院牙周科贵州遵义 563000;
2. 贵州省高等学校口腔疾病研究特色重点实验室

收稿日期:2014-09-24 出版日期:2015-02-28 发布日期:2016-04-29
通讯作者: 刘建国,E-mail:liujg_001@163.com
作者简介:孔宁静(1983~ ),助教,硕士,主要从事牙周病学的研究工作。
基金资助:
贵州省科技创新人才团队建设项目(合同号:黔科合人才团队[2013] 4026号) 贵州省高等学校重点学科建设项目(合同号:SZXK-201207-04) 贵州省高等学校特色重点实验室建设项目(合同号:黔教合KY字[2013]109号) 贵州省科学技术基金项目(合同号:黔科合J字LKZ[2010]50号) 遵义市红花岗区科学技术项目(遵红科合社字[2011]10号）

Effect of Tea Polyphenols on Secretion and Expression of Toll Like Receptor 4 of Human Periodontal Ligament Cells Stimulated by Lipopolysaccharide.

KONG Ning-jing, LI Xiao-na, FAN Qin, et al

School of Stomatology, Zunyi Medical University, Zunyi 563000

Received:2014-09-24 Online:2015-02-28 Published:2016-04-29

摘要/Abstract

摘要： 目的:观察茶多酚(tea polyphenol,TP)对内毒素(lipopolysaccharide,LPS)作用下人牙周膜细胞(periodontal ligament cells, PDLCs)分泌和表达Toll样受体4(toll-like receptor 4,TLR4)的影响。方法:体外分离及培养PDLCs,实验组分别为LPS和不同质量浓度的TP的不同组合,对照组为仅含1% FBS的DMEM培养液。培养24 h、48 h和72 h后,通过酶联免疫吸附测定法检测TLR4的分泌量,荧光实时定量PCR法检测TLR4的表达。结果:100 mg/L LPS组PDLCs TLR4分泌量显著高于其它各组(P<0.05),加入TP进行干预后实验组与对照组TLR4的分泌量和表达量无显著差异(P>0.05)。结论:TP对LPS作用下PDLCs TLR4的分泌和表达有一定的抑制作用。

关键词: 茶多酚, 脂多糖, 人牙周膜细胞, TLR4

Abstract: Objective: To examine the effect of tea polyphonenol (TP) on secretion and expression of toll like receptor 4(TLR4) of human periodontal ligament cells(PDLCs) stimulated by lippolysaccharide. Methods: We isolated PDLCs externally and cultured them. For TP experimental group we used different combination of LPS and TP different concentration, and for the matched group we used DMEM nutrient solutions of 1% FBS. All groups were cultured by 24 h, 48 h and 72 h. The level of TLR4 was examined by ELISA and real-time PCR. Results: 100 μg·mL^-1 LPS can significantly enhance the secretion and expression of TLR4 (P<0.05) , and TP significantly inhibits the secretion and expression of TLR4. Conclusion: TP can induce the secretion and expression of PDLCs stimulated by LPS.

Key words: Tea polyphenols, Lip polysaccharide, Periodontal ligament cells , Toll like receptor 4

中图分类号:

R781.4

孔宁静,李小娜,范芹,管晓燕,白国辉,刘建国. 茶多酚对内毒素作用下人牙周膜细胞分泌和表达Toll样受体4的影响[J]. 口腔医学研究, 2015, 31(2): 140-143.

KONG Ning-jing, LI Xiao-na, FAN Qin, et al. Effect of Tea Polyphenols on Secretion and Expression of Toll Like Receptor 4 of Human Periodontal Ligament Cells Stimulated by Lipopolysaccharide.[J]. Journal of Oral Science Research, 2015, 31(2): 140-143.

参考文献

[1] Madianos PN, Bobetsis YA, Kinane DF. Generation of inflammatory stimuli: how bacteria set up inflammatory responses in the gingival[J]. J Clin Periodontol, 2005, 32(6)∶57-71
[2] 朱光勋,李成章.TOLL样受体与牙周病.国外医学口腔医学分册,2005,32(4)∶285- 287
[3] West AP, Koblansky AA, Ghosh S. Recognition and signaling by toll-like receptors[J]. Annu Rev Cell Dev Biol,2006,22∶409-37
[4] 潘喜华,杨隽,郑勇英, 等.茶多酚调节免疫、抑制肿瘤及抗衰老作用的研究[J].上海预防医学,2000,12(2)∶58-60
[5] Akira S, Takeda K, Kaisho T. Toll-like receptors: critical proteins linking innate and acquired immunity[J]. Nat Immunol, 2001, 2(8)∶675-80
[6] Hirschfeld M, Weis JJ. Signaling by toll-like receptor 2 and 4 agonists results in differential gene expression in murine macrophages[J]. Infect Immun, 2001, 69(3)∶1477-1482
[7] wang PL. Azuma Y, Shinohara M, et al. Toll-like receptor 4- mediated signal pathway induced by Porphyromonas gingivalis lipopolysaccharide in human gingival fibroblasts[J]. Biophys Res Commun, 2000, 273(3)∶1161-1167
[8] Wang PL, Oido-Mori M, Fuji T. THeterogeneous expression of Toll-like receptor 4 and downregulation of Toll-like receptor 4 expression on human gingival fibroblasts by Porphyromonas gingivalis lipopolysaccharide[J]. Biochem Biophys Res Commun, 2001, 288(4)∶863- 867
[9] Hatakeyama J, Tamai R, Sugiyama A, et al. Contrasting responses of human gingival and periodontal ligament fibroblasts to bacterial cell-surface components through the CD14/Toll-like receptor system[J]. Oral Microbiol Immunol, 2003 ,18(1)∶14-23
[10] 孙颖束蓉张秀丽,等.TLR4对人牙周膜细胞分泌IL-1β、IL-6和TNF-α的影响[J]. 上海交通大学学报(医学版),2007,27(6)∶646-648
[11] Kikuchi T, Matsuguchi T, Tsuboi N, et al. Gene expression of osteoclast differentiation factor is induced by lipopolysaccharide in mouse osteoblasts via Toll-like receptors[J]. J Immunol, 2001,166(5)∶3574-3579
[12] 王萍,季平,罗慧琼,等.复方茶多酚含漱液对牙周炎患者龈下菌群及龈沟液内毒素水平的影响[J].重庆医科大学学报,2012,32(1)∶72-75
[13] Cabrera C, Artacho R, Giménez R. Beneficial effects of green tea--a review[J]. J Am Coll Nutr, 2006, 25(2)∶79-99
[14] Sakanaka S1, Okada Y. Inhibitory effects of green tea polyphenols on the production of a virulence factor of the periodontal-disease-causing anaerobic bacterium Porphyromonas gingivalis[J]. J Agric Food Chem, 2004, 52(6)∶1688-92
[15] Youn HS, Lee JY, Saitoh SI, et al. Suppression of MyD88- and TRIF-dependent signaling pathways of Toll-like receptor by(-)-epigallocatechin-3-gallate, a polyphenol component of green tea[J]. Biochem Pharmacol, 2006, 72(7)∶850-859