口腔医学研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (6): 591-593.

• 基础研究论著 • 上一篇    下一篇

大鼠实验性牙周炎治疗前后血清中IL-18与sTREM-1的浓度变化

石丽萍, 何艳艳, 王丽*   

  1. 哈尔滨医科大学附属口腔医院牙周病科 黑龙江 哈尔滨 150001
  • 收稿日期:2014-11-11 出版日期:2015-06-28 发布日期:2016-04-29
  • 通讯作者: 王丽,E-mail:wlidoctor@163.com
  • 作者简介:石丽萍(1989~ ),女,黑龙江人,硕士在读,主要从事牙周疾病的基础研究。

Changes of Serum IL-18 and sTREM－1 Levels in Experimental Periodontitis in Rats Before and After Treatment.

SHI Li-ping, HE Yan-yan, WANG Li*.   

  1. Department of Periodontology, the Affiliatal Stomatological Hospital of Harbin Medical University, Harbin 150001
  • Received:2014-11-11 Online:2015-06-28 Published:2016-04-29

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摘要： 目的:观察大鼠实验性牙周炎基础治疗前后血清中IL-18与sTREM-1水平的变化,进而探讨IL-18与sTREM-1水平变化与牙周炎治疗效果间的关系。方法:选取2个月大的健康雄性Wistar大鼠30只,同等条件下饲养,随机分为3组,每组10只。正常对照组(A组)不做其他处理;牙周炎组(B组)与牙周炎治组(C组),将消毒尼龙丝线放置在双侧上颌第二磨牙牙颈部,同时配合牙周炎食谱诱导大鼠牙周炎模型。建模成功后C组去除丝线进行基础治疗,2周后对大鼠进行腹主动脉取血并处死,采用酶联免疫吸附法(ELISA)测定大鼠血清中IL-18和sTREM-1水平。结果:通过HE染色观察各组牙周组织学改变,牙周炎组(B组)大鼠血清中IL-18及sTREM-1水平高于正常对照组(A组)和牙周炎治疗组(C组)(P<0.05),且牙周炎治疗组大鼠血清中IL-18及sTREM-1水平高于对照组(P<0.05)。结论:牙周炎时IL-18、sTREM-1水平明显增高,且经基础治疗后明显降低。表明IL-18、sTREM-1水平可以为牙周炎的诊断和预测提供指导,为临床工作者提供准确有效的信息,同时也为临床治疗提供新的治疗靶点。

关键词: 大鼠, 牙周炎, IL-18, sTREM-1

Abstract: Objective: To observe the changes of serum IL-18(interleukin18) and sTREM-1(striggering receptor1 expressedon myeloid cells, TREM-1) levels before and after the foundation treatment in rats with periodontitis and explore the relationship between serum IL-18, sTREM-1 levels and response to treatment. Methods: 30 healthy 2 -month-old male Wistar rats were randomly divided into three groups (n=10). The rats with no other processing as the normal controls (Group A). The second premolars of group B and group C were ligated with silk, fed with a periodontitis recipe. After the success of modeling, we removed the silk of C group and then gave basic treatment. After two weeks, abdominal aortic blood and rats were killed, the level of IL-18 and sTREM-1 in serum were measured by ELISA. Results: In untreated periodontitis group the level of IL-18 and sTREM-1 in serum were significantly higher than the control group and the treatment of periodontitis -based group (P<0.05) and the level of IL-18 and sTREM-1 in serum of the treatment of periodontitis-based group was higher than the control group. Conclusion: The level of IL-18 and sTREM-1 in serum significantly increased in periodontitis groups, and obviously reduced after basic treatment. This indicated that the IL-18 and sTREM-1 levels could provide guidance for the diagnosis and prognosis of periodontitis, provide accurate and useful information for clinical workers, also could provide new therapeutic targets for clinical treatment.

Key words: Rat periodontitis , IL-18 , sTREM-1

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