组织蛋白酶K抑制剂对正畸牙移动牙周组织改建的影响

doi:10.13701/j.cnki.kqyxyj.2017.12.003

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (12): 1250-1253.DOI: 10.13701/j.cnki.kqyxyj.2017.12.003

组织蛋白酶K抑制剂对正畸牙移动牙周组织改建的影响

黄鹂,周泽渊,王欣,宿晨曦,邹淑娟^*

四川大学华西口腔医院正畸科四川成都 610041

收稿日期:2017-06-27 出版日期:2017-12-20 发布日期:2018-01-03
通讯作者: 邹淑娟,电话:(028)85501474
作者简介:黄鹂(1992~ ),女,重庆人,硕士在读,主要从事口腔正畸学研究工作。
基金资助:
国家自然科学基金(编号:81470777)

Effect of Cathepsin K Inhibitor on Remodeling of Periodontium during Orthodontic Tooth Movement.

HUANG Li, ZHOU Ze-yuan, WANG Xin, SU Chen-xi, ZOU Shu-juan^*.

Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.

Received:2017-06-27 Online:2017-12-20 Published:2018-01-03

摘要/Abstract

摘要： 目的: 探究组织蛋白酶K抑制剂对大鼠正畸牙移动阶段牙周组织改建的影响。方法: 42只Wistar雄性大鼠,建立正畸牙移动模型,在牙移动第0天时处死6只大鼠作为基线,其余随机分为实验组和对照组,分别给予同剂量的组织蛋白酶K抑制剂和生理盐水局部注射,牙移动后第3、7、14天处死,取上颌骨第一磨牙远中根周围的牙周组织,进行HE染色、组织蛋白酶K及I型胶原免疫组织化学染色。结果: 组织蛋白酶K主要在压力区表达,实验组组织蛋白酶K的阳性表达少于对照组,第7、14天时的表达量差异性显著(P<0.05)。I型胶原主要在张力区表达,实验组I型胶原的阳性表达多于对照组,第7、14天时的表达差异显著(P<0.05)。结论: 组织蛋白酶K抑制剂影响正畸牙移动过程牙周组织的改建,可抑制牙周组织中组织蛋白酶K的表达,促进I型胶原的分泌。

关键词: 正畸牙移动, 组织蛋白酶K抑制剂, Cat-K, COL, I

Abstract: Objective: To investigate the effect of cathepsin K inhibitor on remodeling of periodontium during orthodontic tooth movement. Methods: Forty-two Wistar rats were subjected orthodontic tooth movement for 3, 7 and 14 days. Six rats were sacrificed immediately as initial control, the rest of rats were randomly divided into ODN group received local injection every third day with ODN and control group with saline of the same dose. Samples were collected at the 14th day after tooth movement period. The results were assessed by HE and IHC of Cat-K and COL I. Results: Cat-K mainly expressed in the pressure area and the positive expression of ODN group was less than the control group. COL I mainly expressed in the tension area and the positive expression of ODN group was more than the control group. The IHC results of Cat-K and COL I both had statistical significant difference on the 7th and 14th day. Conclusion: The Cat-K inhibitor odanacatib played a role in remodeling during orthodontic tooth movement. It could inhibit the expression of Cat-K and promote the secretion of COL I.

Key words: Othodontic tooth movement, Cathepsin, K inhibitor, Cat-K, COL I

中图分类号:

R783.5

黄鹂,周泽渊,王欣,宿晨曦,邹淑娟. 组织蛋白酶K抑制剂对正畸牙移动牙周组织改建的影响[J]. 口腔医学研究, 2017, 33(12): 1250-1253.

HUANG Li, ZHOU Ze-yuan, WANG Xin, SU Chen-xi, ZOU Shu-juan.. Effect of Cathepsin K Inhibitor on Remodeling of Periodontium during Orthodontic Tooth Movement.[J]. Journal of Oral Science Research, 2017, 33(12): 1250-1253.

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组织蛋白酶K抑制剂对正畸牙移动牙周组织改建的影响

Effect of Cathepsin K Inhibitor on Remodeling of Periodontium during Orthodontic Tooth Movement.

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