PMX205抗炎作用的体外研究

doi:10.13701/j.cnki.kqyxyj.2017.12.002

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (12): 1246-1249.DOI: 10.13701/j.cnki.kqyxyj.2017.12.002

PMX205抗炎作用的体外研究

李格格¹,唐秋玲¹,潘佳慧¹,王柳然¹,孟阳¹,岳轶云¹,丁小函¹,刘东宁¹,于维先^1,2,3*

1. 吉林大学口腔医学院牙周病科吉林长春 130021;
2. 吉林大学口腔医学院实验教学中心吉林长春 130021;
3. 吉林省牙发育及颌骨重塑与再生重点实验室吉林长春 130021

收稿日期:2017-06-08 出版日期:2017-12-20 发布日期:2018-01-03
通讯作者: 于维先,E-mail:yu-wei-xian@163.com
作者简介:李格格(1990~ ),女,山东人,医师,硕士,主要从事口腔牙周病学临床和基础研究工作。
基金资助:
国家自然科学基金面上项目(编号:81570983)；吉林省卫生技术创新项目(编号:2016J073)；吉林省科技厅自然科学基金项目(编号:20150101076JC)；吉林大学研究生创新基金资助项目(编号:2017130)

Study on Anti-inflammatory Effects of PMX205 in Vitro

LI Ge-ge¹, TANG Qiu-ling¹, PAN Jia-hui¹, WANG Liu-ran¹, MENG Yang¹, YUE Yi-yun¹, DING Xiao-han¹, LIU Dong-ning¹, YU Wei-xian^1,2,3*

1. Department of Periodontics, School of Stomatology, Jilin University, Changchun 130021, China;
2. Experimental Teaching Center, School of Stomatology, Jilin University, Changchun 130021, China;
3. Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Changchun 130021, China.

Received:2017-06-08 Online:2017-12-20 Published:2018-01-03

摘要/Abstract

摘要： 目的: 探讨补体C5a受体拮抗剂PMX205的体外抗炎作用。方法: MTT法检测PMX205对小鼠巨噬细胞(RAW264.7)的毒性。RAW264.7与牙龈蛋白酶(gingipain,G)体外共培养模拟炎症环境,观察PMX205的抗炎效果。实验分为6组:阴性对照组、G组、G+PMX205(0.1、1、5、10 mg/L)组,24 h后qRT-PCR、ELISA法检测M1型巨噬细胞标志性细胞因子IL-6及TNF-α,M2型标志性细胞因子IL-10和TGF-β1;流式细胞术检测M1型标志性分子CD86及M2型标志性分子CD206。结果: MTT结果显示,PMX205对RAW264.7活力影响较小。qRT-PCR及ELISA结果显示,G组IL-6和TNF-α表达水平较阴性对照组增加;G+PMX205各浓度组的IL-6和TNF-α的表达水平较G组减少,TGF-β1和IL-10表达水平均较G组增加(P＜0.01或P<0.05)。流式细胞术结果显示,G组CD86阳性率增加,CD206阳性率下降,G+PMX205组较G组CD86下降,而CD206增加。结论: 在体外细胞模型实验中PMX205具有良好的抗炎作用。

关键词: 牙周炎, 牙龈蛋白酶, PMX205, 巨噬细胞, 极化

Abstract: Objective: To explore the anti-inflammatory effects of C5aR antagonist PMX205. Methods: The toxicity of PMX205 for the mouse macrophage (RAW264.7) was tested with MTT assay. RAW264.7 cells were co-cultured with gingipains (G) to simulate the inflammatory environment in vitro and the anti-inflammatory effects of PMX205 was observed. The experiment was divided into six groups: negative control group, G group, and G + PMX205(0.1,1,5,10 mg/L)group. After cultured for 24 h, qRT-PCR and ELISA were used to detect IL-6, TNF-α, IL-10 and TGF-β1. Flow cytometry was used to detect the M1 marker molecule CD86 and M2 marker molecule CD206. Results: MTT results showed that PMX205 had little effect on the viability of RAW264.7. qRT-PCR and ELISA results showed that IL-6 and TNF-α expression levels of G group were higher than the negative control group. The expression of IL-6 and TNF-α were decreased in G + PMX205 group, and TGF-β1 and IL-10 were higher than those in G group. Flow cytometry showed that CD86 positive rate was increased and the CD206 positive rate was decreased in G group. In G+PMX205 group the CD86 was decreased, the CD206 was higher than those in G group. Conclusion: In vitro cell model experiments, PMX205 showed good anti-inflammatory effects

Key words: Periodontitis , Gingipain , PMX205, Macrophage , Polarization

中图分类号:

R781.4

李格格,唐秋玲,潘佳慧,王柳然,孟阳,岳轶云,丁小函,刘东宁,于维先. PMX205抗炎作用的体外研究[J]. 口腔医学研究, 2017, 33(12): 1246-1249.

LI Ge-ge, TANG Qiu-ling, PAN Jia-hui, WANG Liu-ran, MENG Yang, YUE Yi-yun, DING Xiao-han, LIU Dong-ning, YU Wei-xian. Study on Anti-inflammatory Effects of PMX205 in Vitro[J]. Journal of Oral Science Research, 2017, 33(12): 1246-1249.

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PMX205抗炎作用的体外研究

Study on Anti-inflammatory Effects of PMX205 in Vitro

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