口腔医学研究

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (5): 567-571.DOI: 10.13701/j.cnki.kqyxyj.2018.05.025

• 其他研究 • 上一篇    下一篇

口腔角化软组织缺损兔模型的建立与评估

王磊1, 范亚伟2*   

  1. 1. 山西医科大学口腔医学系 山西 太原 030001;
    2. 山西医科大学第一医院口腔科 山西 太原 030001
  • 收稿日期:2017-11-09 出版日期:2018-05-28 发布日期:2018-05-29
  • 通讯作者: 范亚伟,E-mail:yaweifan1970@163.com
  • 作者简介:王磊(1990~ ),男,重庆人,硕士在读,主要从事口腔种植体周围软组织的研究。

Establishment and Evaluation of A Rabbit Model of Oral Keratinized Soft Tissue Wound.

WANG Lei1, FANG Ya-wei2*   

  1. 1. School of Stomatology, Shanxi Medical University, Taiyuan 030001, China;
    2. Department of Stomatology, First Hospital of Shanxi Medical University, Taiyuan 030001, China
  • Received:2017-11-09 Online:2018-05-28 Published:2018-05-29

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被引次数

2

摘要： 目的:构建一个具有科学性、重复性、相似性、可靠性、标准化的口腔角化软组织缺损动物模型,并对其形态学和组织学特征进行评估。方法:12只3月龄雄性日本大耳白兔,随机分为3、7、14 d和21 d组(n=3)。在硬腭中1/3中份,距上颌牙10 mm和两侧黏膜边缘2.5 mm处制备10 mm角化黏膜缺损。术中随机挑选三块切取下来的组织块作为对照组。测量各组缺损组织块的平均厚度,排除因其厚度不同所带来的误差。分别于术后3、7、14、21 d进行大体观察和创面愈合率分析,并于术区取材后行HE染色和VG染色,观察其炎症反应、上皮的修复和胶原纤维的合成状况。结果:大体观察:3 d炎症最重,随后炎症逐渐减轻,缺损组织逐渐恢复。组织学观察:3 d炎症细胞最多,术后7 d,细胞不断增殖,肉芽组织大量形成,角化上皮和纤维结缔组织开始逐渐恢复。各组缺损的平均厚度无统计学差异。愈合率:3 d<7 d<14 d<21 d(P<0.01)。炎症等级评分:3 d和7 d分别都高于对照组(P<0.05)。上皮平均厚度:14 d小于对照组和21 d(P<0.05)。胶原纤维的密度评分:3 d和7 d都低于对照组(P<0.05),21 d高于对照组(P<0.05)。结论:成功建立了口腔角化软组织缺损动物模型,为后续研究促进口腔角化软组织修复的方法、材料和机制奠定了基础。

关键词: 创伤愈合, 动物模型, 兔, 硬腭, 结缔组织

Abstract: Objective: To establish an animal model of oral keratinized soft tissue wound with scientificalness, repeatability, comparability, reliability, and standardization, and to evaluate its morphology and histology. Methods: 3-month-old male Japanese rabbits were divided randomly into 4 groups: 3d, 7d, 14d and 21d groups (n=3). Keratinized soft tissue wound was generated by using a punch-biopsy instrument (diameter 10mm) at the middle third of hard palate, respectively far from bilateral mucosal margin 2.5mm, and away from maxillary teeth 10mm. Three soft tissues were randomly chosen and cut as control group. Gross observation and wound healing analysis were performed after 3, 7, 14, and 21 days. HE staining and VG staining were used to observe inflammatory reaction, healing of the epithelial thickness, and collagen formation, after collected the specimen on the surgery area. Results: Gross observation: the inflammatory reaction after 3 days was the most severe. As time went by, the wound was gradually healed. Histological observation: the number of inflammatory cells was the most after 3 days. Keratinized epithelium and fibrous connective tissue were gradually healed after 7 days, with cell proliferation and formation of granulation tissue. The wound thickness in each group had no statistical difference. The wound healing rate: 3d< 7d<14d<21d (P<0.01). The grades of inflammatory reaction of both 3d and 7d groups were higher than those of control group, 14d, and 21d groups (P<0.05). The average epithelial thickness of 14d group was narrower than those of control group and 21d group (P<0.05). The grades of the density of collagen fibres of both 3d and 7d groups were lower than that of control group (P<0.05), and that of 21d group was higher than that of control group (P<0.05). Conclusion: An animal model of oral keratinized soft tissue wound was successfully established, which laid a foundation for researching the way, the material, and the mechanism of promoting healing of oral keratinized soft tissue in the future.

Key words: Wound healing, Animal model, Rabbits, Hard palate, Connective tissue