超重小鼠应对牙周感染的血清炎症因子反应

doi:10.13701/j.cnki.kqyxyj.2018.06.017

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (6): 644-647.DOI: 10.13701/j.cnki.kqyxyj.2018.06.017

超重小鼠应对牙周感染的血清炎症因子反应

余挺¹, 赵莉², 章锦才³, 轩东英^4*

1. 广州医科大学附属口腔医院·广州口腔疾病研究所·口腔医学重点实验室牙周科广东广州 510140;
2. 中山大学光华口腔医学院·附属口腔医院,广东省口腔医学重点实验室,口腔修复科广东广州 510055;
3. 中国科学院大学·存济医学院北京 100049;
4. 中国科学院大学存济医学院,杭州口腔医院牙周科浙江杭州 310006

收稿日期:2017-12-01 出版日期:2018-06-20 发布日期:2018-06-21
通讯作者: 轩东英,E-mail: xuanxuan187@126.com
作者简介:余挺(1987~ ),男,博士,医师,主要从事牙周病学的临床和科研工作。
基金资助:
国家自然科学基金(编号:81700985、81371151、81470750);广州市卫生和计划生育科技项目(编号:20181A011102)

Serum Cytokine Response to Periodontal Infection in Overweight Mice

YU Ting¹, ZHAO Li², ZHANG Jin-cai³, XUAN Dong-ying^4*

1. Department of Periodontology, Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou 510150, China;
2. Department of Prosthodontics, Guanghua School of Stomatology, Sun Yat-sen University and Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China;
3. Savaid Medical School, University of Chinese Academy of Sciences, Beijing 100049, China;
4. Department of Periodontology, Hangzhou Dental Hospital, Savaid Medical School, University of Chinese Academy of Sciences, Hangzhou 310006, China

Received:2017-12-01 Online:2018-06-20 Published:2018-06-21

摘要/Abstract

摘要： 目的:探索超重宿主应对牙周感染的系统性免疫反应。方法:将28只C57 BL/6J小鼠分为高脂(HFD)和低脂饲料(LFD)组,HFD诱导超重模型,LFD作为正常体重对照。诱导8周时,两饲料组均再分为牙周炎(P)和健康(C)组(n=7),P组做牙周结扎,C组做对照。10 d后处死,Micro-CT分析骨丧失线性分数,蛋白芯片筛检血清中20个炎症因子水平,单因素方差分析比较4组间差异。结果:HFD组的体重高于LFD组3.8 g(15.5%)(P<0.01)。P组的骨丧失线性分数显著高于C组(P< 0.01)。除HFDP组的 IL-6水平较LFDC组显著上调外(P<0.05),其他炎症因子反应在HFD与LFD组间无显著差异,在HFD小鼠的P组与C组间亦无差异。结论:未发现系统性炎症介导超重与牙周炎的相互关系。

关键词: 肥胖, 超重, 牙周炎, 炎症, 细胞因子

Abstract: Objective: To explore systemic immune response of overweight host to periodontal infection. Methods: Twenty-eight C57 BL/6J mice were divided into high-(HFD) or low-fat diet (LFD) groups to induce overweight or being as controls, respectively. After 8 weeks on diet, both two diet groups were subdivided into periodontitis (P) or periodontal-health controls (C) (n=7), with the P group for periodontal ligation and the C group as controls. The mice were killed after 10 days’ ligation. Linear bone loss fraction was analyzed by micro-CT. Twenty candidate serum cytokines were detected by protein arrays. Differences between the four groups were analyzed by one-way ANOVA. Results: The HFD group had 3.8g or 15.5% higher body weight than that of the LFD group (P<0.01). The P group had significantly higher linear bone loss fraction compared with that of the C group (P<0.01). Except for IL-6, the level of which was significantly higher in the HFDP group relative to the LFDC group (P<0.05), no any other serum cytokines showed significantly different levels between HFD and LFD groups or between P and C groups within the HFD mice. Conclusion: No significant mediating role of systemic inflammation is found in correlation between overweight and periodontitis.

Key words: Obesity, Overweight, Periodontal diseases, Inflammation, Cytokine

余挺, 赵莉, 章锦才, 轩东英. 超重小鼠应对牙周感染的血清炎症因子反应[J]. 口腔医学研究, 2018, 34(6): 644-647.

YU Ting, ZHAO Li, ZHANG Jin-cai, XUAN Dong-ying. Serum Cytokine Response to Periodontal Infection in Overweight Mice[J]. Journal of Oral Science Research, 2018, 34(6): 644-647.

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超重小鼠应对牙周感染的血清炎症因子反应

Serum Cytokine Response to Periodontal Infection in Overweight Mice

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