MicroRNA-9影响涎腺腺样囊性癌侵袭能力的分子机制研究

doi:10.13701/j.cnki.kqyxyj.2019.06.013

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (6): 563-567.DOI: 10.13701/j.cnki.kqyxyj.2019.06.013

MicroRNA-9影响涎腺腺样囊性癌侵袭能力的分子机制研究

徐川^1,2,李亚楠¹,谯丹¹,古欢¹,钟罗¹,吴键¹,于涛^1,2*

1. 电子科技大学附属肿瘤医院头颈外科四川成都 610041;
2. 四川省肿瘤医院头颈外科四川成都 610041

收稿日期:2019-03-16 出版日期:2019-06-28 发布日期:2019-06-27
通讯作者: 于涛,E-mail:vince8181@163.com
作者简介:徐川(1964~ ),男,浙江宁波人,副主任医师,主要从事口腔癌分子机制的相关研究。
基金资助:
国家自然科学基金(编号:81602387)
四川省科技计划重点研发项目(编号:2018SZ0154)
四川省应用基础研究(编号:2019YJ0572)

Molecular Mechanism of MicroRNA-9 Influencing Invasion of Salivary Adenoid Cystic Carcinoma Cells

XU Chuan^1,2, LI Ya-nan¹, QIAO Dan¹, GU Huan¹, ZHONG Luo¹, WU Jian¹, YU Tao^1,2*

1. Department of Head and Neck, the Affiliated Cancer Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610041, China;
2. Department of Head and Neck, Sichuan Cancer Hospital &Institute, Chengdu 610041, China.

Received:2019-03-16 Online:2019-06-28 Published:2019-06-27

摘要/Abstract

摘要： 目的:探讨miR-9对人涎腺腺样囊性癌细胞侵袭的影响及其相关分子机制。方法:检测ACC-2,ACC-M细胞中miR-9表达,构建慢病毒miR-9过表达载体,转染ACC-2,ACC-M肿瘤细胞,实时荧光定量PCR检测细胞中 miR-9的表达水平。Transwell方法检测转染miR-9前后ACC-M细胞侵袭转移能力的变化,Western 免疫印迹检测转染前后ACC-M细胞中基质金属蛋白酶家族蛋白(MMP)及数个肿瘤相关信号通路蛋白的表达情况,采用 SPSS 16.0 软件包对数据进行统计学分析。结果:相比于ACC-2,ACC-M细胞中出现了显著的miR-9的表达下调(P<0.01)。携带miR-9的慢病毒转染细胞后均显著提高了2株细胞中miR-9的表达并显著降低了ACC-M细胞的侵袭能力(P<0.01)。同时,转染miR-9后显著降低了MMP-2及MMP-9蛋白表达及AKT蛋白的磷酸化水平。结论:上调miR-9 的表达可显著抑制ACC-M细胞的侵袭能力,其机制可能与AKT信号通路介导的MMP-2,9蛋白的表达下调密切相关。

关键词: 腺样囊性癌, 微小RNA, 基质金属蛋白酶, AKT信号通路

Abstract: Objective: To investigate the effect and regulatory mechanisms of microRNA-9 on invasion of human salivary adenoid cystic carcinoma (SACC) cells. Methods: The expressions of microRNA-9 (miR-9) from ACC-2 cells and ACC-M cells were analyzed using qRT-PCR after transfected with miR-9 lentivirus. Cell invasion was measured by transwell experiment after transfection. Western blot was used to detect the protein expression levels of matrix metalloproteinases family and tumor-related signaling pathways in SACC cell lines after transfection. SPSS 16.0 was used to analyze the data. Results: MiR-9 expression was remarkably down-regulated in the ACC-M cells in contrast to ACC-2 cells (P<0.01). After 72h transfection, the expression of miR-9 was increased in both ACC cells (P<0.01). MiR-9 overexpression triggered to a decreased cell invasion after transfection for ACC-M cells (P<0.01). The expressions of MMP-2 and MMP-9 proteins were significantly decreased after transfection in the ACC-M cells, while AKT protein phosphorylation was significantly decreased. Conclusion: Upregulation of miR-9 can inhibit the invasion of SACC cells. The mechanism might relate to the down-regulation of MMP-2 and MMP-9 mediated by AKT signaling pathway.

Key words: Adenoid cystic carcinoma, MicroRNA, Matrix metalloproteinase, AKT signaling pathway

徐川,李亚楠,谯丹,古欢,钟罗,吴键,于涛. MicroRNA-9影响涎腺腺样囊性癌侵袭能力的分子机制研究[J]. 口腔医学研究, 2019, 35(6): 563-567.

XU Chuan, LI Ya-nan, QIAO Dan, GU Huan, ZHONG Luo, WU Jian, YU Tao. Molecular Mechanism of MicroRNA-9 Influencing Invasion of Salivary Adenoid Cystic Carcinoma Cells[J]. Journal of Oral Science Research, 2019, 35(6): 563-567.

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MicroRNA-9影响涎腺腺样囊性癌侵袭能力的分子机制研究

Molecular Mechanism of MicroRNA-9 Influencing Invasion of Salivary Adenoid Cystic Carcinoma Cells

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